ARG42510
anti-RAB5A antibody
anti-RAB5A antibody for ICC/IF,Western blot and Human,Mouse,Rat,Dog,Monkey
Overview
Product Description | Goat Polyclonal antibody recognizes RAB5A |
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Tested Reactivity | Hu, Ms, Rat, Dog, Mk |
Tested Application | ICC/IF, WB |
Host | Goat |
Clonality | Polyclonal |
Isotype | IgG |
Target Name | RAB5A |
Antigen Species | Mouse |
Immunogen | Purified recombinant peptide within aa. 115 to the C-terminus of Mouse RAB5A. |
Conjugation | Un-conjugated |
Alternate Names | Ras-related protein Rab-5A; RAB5 |
Application Instructions
Application Suggestion |
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Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||
Positive Control | SW948, NIH/3T3, MCF7, MDCK and HeLa | ||||||
Observed Size | ~ 27 kDa |
Properties
Form | Liquid |
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Purification | Affinity purification with immunogen. |
Buffer | PBS, 0.05% Sodium azide and 20% Glycerol. |
Preservative | 0.05% Sodium azide |
Stabilizer | 20% Glycerol |
Concentration | 4 mg/ml |
Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
Database Links | |
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Gene Symbol | RAB5A |
Gene Full Name | RAB5A, member RAS oncogene family |
Function | Small GTPase which cycles between active GTP-bound and inactive GDP-bound states. In its active state, binds to a variety of effector proteins to regulate cellular responses such as of intracellular membrane trafficking, from the formation of transport vesicles to their fusion with membranes. Active GTP-bound form is able to recruit to membranes different sets of downstream effectors directly responsible for vesicle formation, movement, tethering and fusion. RAB5A is required for the fusion of plasma membranes and early endosomes (PubMed:10818110, PubMed:14617813, PubMed:16410077, PubMed:15378032). Contributes to the regulation of filopodia extension (PubMed:14978216). Required for the exosomal release of SDCBP, CD63, PDCD6IP and syndecan (PubMed:22660413). Regulates maturation of apoptotic cell-containing phagosomes, probably downstream of DYN2 and PIK3C3 (By similarity). [UniProt] |
Cellular Localization | Cell membrane; Lipid-anchor; Cytoplasmic side. Early endosome membrane; Lipid-anchor. Melanosome. Cytoplasmic vesicle. Cell projection, ruffle. Membrane. Cytoplasm, cytosol. Cytoplasmic vesicle, phagosome membrane. Endosome membrane. Note=Enriched in stage I melanosomes (PubMed:17081065). Alternates between membrane-bound and cytosolic forms (Probable). [UniProt] |
Calculated MW | 24 kDa |
Images (2) Click the Picture to Zoom In
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ARG42510 anti-RAB5A antibody ICC/IF image
Immunofluorescence: Hepa1-6 cells were fixed with 4% PFA. Cells were stained with ARG42510 anti-RAB5A antibody (green) at 1:50 dilution. Nuclear staining (blue).
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ARG42510 anti-RAB5A antibody WB image
Western blot: 50 µg of SW948, NIH/3T3, MCF7, MDCK and HeLa cell lysates stained with ARG42510 anti-RAB5A antibody at 1:2500 dilution.