ARG42510

anti-RAB5A antibody

anti-RAB5A antibody for ICC/IF,Western blot and Human,Mouse,Rat,Dog,Monkey

Overview

Product Description Goat Polyclonal antibody recognizes RAB5A
Tested Reactivity Hu, Ms, Rat, Dog, Mk
Tested Application ICC/IF, WB
Host Goat
Clonality Polyclonal
Isotype IgG
Target Name RAB5A
Antigen Species Mouse
Immunogen Purified recombinant peptide within aa. 115 to the C-terminus of Mouse RAB5A.
Conjugation Un-conjugated
Alternate Names Ras-related protein Rab-5A; RAB5

Application Instructions

Application Suggestion
Tested Application Dilution
ICC/IF1:50 - 1:250
WB1:250 - 1:5000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Positive Control SW948, NIH/3T3, MCF7, MDCK and HeLa
Observed Size ~ 27 kDa

Properties

Form Liquid
Purification Affinity purification with immunogen.
Buffer PBS, 0.05% Sodium azide and 20% Glycerol.
Preservative 0.05% Sodium azide
Stabilizer 20% Glycerol
Concentration 4 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 271457 Mouse RAB5A

GeneID: 5868 Human RAB5A

GeneID: 64633 Rat RAB5A

Gene Symbol RAB5A
Gene Full Name RAB5A, member RAS oncogene family
Function Small GTPase which cycles between active GTP-bound and inactive GDP-bound states. In its active state, binds to a variety of effector proteins to regulate cellular responses such as of intracellular membrane trafficking, from the formation of transport vesicles to their fusion with membranes. Active GTP-bound form is able to recruit to membranes different sets of downstream effectors directly responsible for vesicle formation, movement, tethering and fusion. RAB5A is required for the fusion of plasma membranes and early endosomes (PubMed:10818110, PubMed:14617813, PubMed:16410077, PubMed:15378032). Contributes to the regulation of filopodia extension (PubMed:14978216). Required for the exosomal release of SDCBP, CD63, PDCD6IP and syndecan (PubMed:22660413). Regulates maturation of apoptotic cell-containing phagosomes, probably downstream of DYN2 and PIK3C3 (By similarity). [UniProt]
Cellular Localization Cell membrane; Lipid-anchor; Cytoplasmic side. Early endosome membrane; Lipid-anchor. Melanosome. Cytoplasmic vesicle. Cell projection, ruffle. Membrane. Cytoplasm, cytosol. Cytoplasmic vesicle, phagosome membrane. Endosome membrane. Note=Enriched in stage I melanosomes (PubMed:17081065). Alternates between membrane-bound and cytosolic forms (Probable). [UniProt]
Calculated MW 24 kDa

Images (2) Click the Picture to Zoom In

  • ARG42510 anti-RAB5A antibody ICC/IF image

    Immunofluorescence: Hepa1-6 cells were fixed with 4% PFA. Cells were stained with ARG42510 anti-RAB5A antibody (green) at 1:50 dilution. Nuclear staining (blue).

  • ARG42510 anti-RAB5A antibody WB image

    Western blot: 50 µg of SW948, NIH/3T3, MCF7, MDCK and HeLa cell lysates stained with ARG42510 anti-RAB5A antibody at 1:2500 dilution.