ARG51436
anti-RAB5 antibody
anti-RAB5 antibody for Western blot and Human,Mouse,Rat
Controls and Markers antibody; Neuroscience antibody; Signaling Transduction antibody
1
Overview
| Product Description | Rabbit Polyclonal antibody recognizes RAB5 |
|---|---|
| Tested Reactivity | Hu, Ms, Rat |
| Tested Application | WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | RAB5 |
| Antigen Species | Human |
| Immunogen | Peptide sequence around aa.188~192( N-P-G-A-N) derived from Human Rab5. |
| Conjugation | Un-conjugated |
| Alternate Names | Ras-related protein Rab-5A; RAB5 |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Antibodies were produced by immunizing rabbits with KLH-conjugated synthetic peptide. Antibodies were purified by affinity-chromatography using epitope-specific peptide. |
| Buffer | PBS (without Mg2+ and Ca2+, pH 7.4), 150mM NaCl, 0.02% Sodium azide and 50% Glycerol. |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 50% Glycerol |
| Concentration | 1 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | RAB5A |
| Gene Full Name | RAB5A, member RAS oncogene family |
| Background | Required for the fusion of plasma membranes and early endosomes. |
| Function | The small GTPases Rab are key regulators of intracellular membrane trafficking, from the formation of transport vesicles to their fusion with membranes. Rabs cycle between an inactive GDP-bound form and an active GTP-bound form that is able to recruit to membranes different sets of downstream effectors directly responsible for vesicle formation, movement, tethering and fusion. RAB5A is required for the fusion of plasma membranes and early endosomes. Contributes to the regulation of filopodia extension. [UniProt] |
| Research Area | Controls and Markers antibody; Neuroscience antibody; Signaling Transduction antibody |
| Calculated MW | 24 kDa |
Images (1) Click the Picture to Zoom In
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ARG51436 anti-RAB5 antibody WB image
Western blot: 30 µg of HeLa and Jurkat cell lysates stained with ARG51436 anti-RAB5 antibody at 1:500 dilution.
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Review for anti-RAB5 antibody
Application：WB
Sample：HeLa and Jurkat
Sample Loading Amount：30 µg
Primary Antibody Dilution Factor：1:500
Primary Antibody Incubation Time：overnight
Primary Antibody Incubation Temperature：4 ºC
