ARG22296
anti-RAB4 antibody
anti-RAB4 antibody for ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat
Overview
| Product Description | Rabbit Polyclonal antibody recognizes RAB4 |
|---|---|
| Tested Reactivity | Hu, Ms, Rat |
| Tested Application | ICC/IF, IHC-P, WB |
| Specificity | Detects ~ 26 kDa. |
| Host | Rabbit |
| Clonality | Polyclonal |
| Target Name | RAB4 |
| Antigen Species | Human |
| Immunogen | C-terminal peptide from Human RAB4 |
| Conjugation | Un-conjugated |
| Alternate Names | Ras-related protein Rab-4A; HRES1; RAB4; HRES-1; HRES-1/RAB4 |
Application Instructions
| Application Suggestion |
|
||||||||
|---|---|---|---|---|---|---|---|---|---|
| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purification with immunogen. |
| Buffer | PBS (pH 7.4), 0.09% Sodium azide and 50% Glycerol |
| Preservative | 0.09% Sodium azide |
| Stabilizer | 50% Glycerol |
| Concentration | 1 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | RAB4A |
| Gene Full Name | RAB4A, member RAS oncogene family |
| Background | This gene is a member of the largest group in the Ras superfamily of small GTPases, which regulate membrane trafficking. The encoded protein is associated with early endosomes and is involved in their sorting and recycling. The protein also plays a role in regulating the recycling of receptors from endosomes to the plasma membrane. Alternatively spliced transcript variants have been observed for this gene. [provided by RefSeq, Dec 2012] |
| Function | Protein transport. Probably involved in vesicular traffic (By similarity). [UniProt] |
| Cellular Localization | Cytoplasm, Membrane |
| Calculated MW | 24 kDa |
| PTM | Phosphorylated by CDK1 kinase during mitosis. |
Images (4) Click the Picture to Zoom In
-
ARG22296 anti-RAB4 antibody ICC/IF image
Immunocytochemistry: 2% Formaldehyde (20 min at RT) fixed Heat Shocked HeLa cells stained with ARG22296 anti-RAB4 antibody (yellow) at 1:150 dilution (12 hours at 4°C). Counterstain: DAPI (blue) nuclear stain at 1:40000 for 120 min at RT. Magnification: 100x. Left: DAPI (blue) nuclear stain, Middle: Primary antibody, Right: Composite.
-
ARG22296 anti-RAB4 antibody IHC image
Immunohistochemistry: Bouin solution fixed Mouse backskin stained with ARG22296 anti-RAB4 antibody (green) at 1:100 dilution (1 hour).
-
ARG22296 anti-RAB4 antibody WB image
Western blot: Human HeLa cell lysates stained with ARG22296 anti-RAB4 antibody at 1:1000 dilution.
-
ARG22296 anti-RAB4 antibody ICC/IF image
Immunocytochemistry: 2% Formaldehyde (20 min at RT) fixed Heat Shocked HeLa cells stained with ARG22296 anti-RAB4 antibody (red) at 1:150 dilution (12 hours at 4°C). Counterstain: DAPI (blue) nuclear stain at 1:40000 for 120 min at RT. Magnification: 20x. Left: DAPI (blue) nuclear stain, Middle: Primary antibody, Right: Composite.
