ARG41356
anti-RAB11B antibody
anti-RAB11B antibody for Flow cytometry,ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat
Overview
| Product Description | Rabbit Polyclonal antibody recognizes RAB11B |
|---|---|
| Tested Reactivity | Hu, Ms, Rat |
| Tested Application | FACS, ICC/IF, IHC-P, WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | RAB11B |
| Antigen Species | Human |
| Immunogen | Synthetic peptide corresponding to a sequence of Human RAB11B. (QKQIADRAAHDESPGNNVVDISVPPTTDGQKPNKLQCC) |
| Conjugation | Un-conjugated |
| Alternate Names | GTP-binding protein YPT3; H-YPT3; Ras-related protein Rab-11B |
Application Instructions
| Application Suggestion |
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| Application Note | IHC-P: Antigen Retrieval: Heat mediation was performed in Citrate buffer (pH 6.0, epitope retrieval solution) for 20 min. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
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| Observed Size | ~ 24 kDa |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purification with immunogen. |
| Buffer | 0.2% Na2HPO4, 0.9% NaCl, 0.05% Sodium azide and 4% Trehalose. |
| Preservative | 0.05% Sodium azide |
| Stabilizer | 4% Trehalose |
| Concentration | 0.5 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
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| Gene Symbol | RAB11B |
| Gene Full Name | RAB11B, member RAS oncogene family |
| Background | The Ras superfamily of small GTP-binding proteins, which includes the Ras (see MIM 190020), Ral (see MIM 179550), Rho (see MIM 165390), Rap (see MIM 179520), and Rab (see MIM 179508) families, is involved in controlling a diverse set of essential cellular functions. The Rab family, including RAB11B, appears to play a critical role in regulating exocytotic and endocytotic pathways (summary by Zhu et al., 1994 [PubMed 7811277]).[supplied by OMIM, Nov 2010] |
| Function | The small GTPases Rab are key regulators of intracellular membrane trafficking, from the formation of transport vesicles to their fusion with membranes. Rabs cycle between an inactive GDP-bound form and an active GTP-bound form that is able to recruit to membranes different set of downstream effectors directly responsible for vesicle formation, movement, tethering and fusion. That Rab plays a role in endocytic recycling, regulating apical recycling of several transmembrane proteins including cystic fibrosis transmembrane conductance regulator/CFTR, epithelial sodium channel/ENaC, potassium voltage-gated channel, and voltage-dependent L-type calcium channel. May also regulate constitutive and regulated secretion, like insulin granule exocytosis. Required for melanosome transport and release from melanocytes. Also regulates V-ATPase intracellular transport in response to extracellular acidosis. [UniProt] |
| Cellular Localization | Recycling endosome membrane; Lipid-anchor; Cytoplasmic side. Cytoplasmic vesicle, secretory vesicle, synaptic vesicle membrane; Lipid-anchor; Cytoplasmic side. Cytoplasmic vesicle, phagosome membrane; Lipid-anchor; Cytoplasmic side. Note=Recruited to phagosomes containing S.aureus. [UniProt] |
| Calculated MW | 24 kDa |
| PTM | Citrullinated by PADI4. [UniProt] |
Images (8) Click the Picture to Zoom In
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ARG41356 anti-RAB11B antibody ICC/IF image
Immunofluorescence: A431 cells stained with ARG41356 anti-RAB11B antibody. DAPI (blue) for nuclear staining.
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ARG41356 anti-RAB11B antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human oesophagus squama cancer tissue. Antigen Retrieval: Heat mediation was performed in Citrate buffer (pH 6.0, epitope retrieval solution) for 20 min. The tissue section was blocked with 10% goat serum. The tissue section was then stained with ARG41356 anti-RAB11B antibody at 1 µg/ml dilution, overnight at 4°C.
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ARG41356 anti-RAB11B antibody WB image
Western blot: 50 µg of samples under reducing conditions. Rat spleen, Rat lung, Rat ovary, Rat kidney, Mouse lung, Mouse ovary, Mouse kidney and Mouse SP20 whole cell lysates stained with ARG41356 anti-RAB11B antibody at 0.5 µg/ml dilution, overnight at 4°C.
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ARG41356 anti-RAB11B antibody FACS image
Flow Cytometry: A549 cells were blocked with 10% normal goat serum and then stained with ARG41356 anti-RAB11B antibody (blue) at 1 µg/10^6 cells for 30 min at 20°C, followed by incubation with DyLight®488 labelled secondary antibody. Isotype control antibody (green) was Rabbit IgG (1 µg/10^6 cells) used under the same conditions. Unlabelled sample (red) was also used as a control.
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ARG41356 anti-RAB11B antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human ovary cancer tissue. Antigen Retrieval: Heat mediation was performed in Citrate buffer (pH 6.0, epitope retrieval solution) for 20 min. The tissue section was blocked with 10% goat serum. The tissue section was then stained with ARG41356 anti-RAB11B antibody at 1 µg/ml dilution, overnight at 4°C.
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ARG41356 anti-RAB11B antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Mouse ovary tissue. Antigen Retrieval: Heat mediation was performed in Citrate buffer (pH 6.0, epitope retrieval solution) for 20 min. The tissue section was blocked with 10% goat serum. The tissue section was then stained with ARG41356 anti-RAB11B antibody at 1 µg/ml dilution, overnight at 4°C.
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ARG41356 anti-RAB11B antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Rat ovary tissue. Antigen Retrieval: Heat mediation was performed in Citrate buffer (pH 6.0, epitope retrieval solution) for 20 min. The tissue section was blocked with 10% goat serum. The tissue section was then stained with ARG41356 anti-RAB11B antibody at 1 µg/ml dilution, overnight at 4°C.
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ARG41356 anti-RAB11B antibody WB image
Western blot: 50 µg of samples under reducing conditions. Human placenta, A549, U-87 MG, PC-3, HeLa, Caco-2 and HL-60 whole cell lysates stained with ARG41356 anti-RAB11B antibody at 0.5 µg/ml dilution, overnight at 4°C.
