Overview

Product Description	Rabbit Polyclonal antibody recognizes RAB11A / YL8
Tested Reactivity	Hu, Ms, Rat
Tested Application	IP, WB
Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Target Name	RAB11A / YL8
Antigen Species	Human
Immunogen	Synthetic peptide of Human RAB11A / YL8.
Conjugation	Un-conjugated
Alternate Names	Rab-11; Ras-related protein Rab-11A; YL8

Application Instructions

Application Suggestion	Tested Application Dilution IP 1:30 WB 1:1000 - 1:2000
Application Note	* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Positive Control	HeLa
Observed Size	~ 24 kDa

Properties

Form	Liquid
Purification	Affinity purified.
Buffer	PBS (pH 7.4), 150 mM NaCl, 0.02% Sodium azide and 50% Glycerol.
Preservative	0.02% Sodium azide
Stabilizer	50% Glycerol
Storage Instruction	For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note	For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links	GeneID: 53869 Mouse RAB11A GeneID: 81830 Rat RAB11A GeneID: 8766 Human RAB11A Swiss-port # P62491 Human Ras-related protein Rab-11A Swiss-port # P62492 Mouse Ras-related protein Rab-11A Swiss-port # P62494 Rat Ras-related protein Rab-11A More
Gene Symbol	RAB11A
Gene Full Name	RAB11A, member RAS oncogene family
Background	The protein encoded by this gene belongs to the Rab family of the small GTPase superfamily. It is associated with both constitutive and regulated secretory pathways, and may be involved in protein transport. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, May 2011]
Function	The small GTPases Rab are key regulators of intracellular membrane trafficking, from the formation of transport vesicles to their fusion with membranes. Rabs cycle between an inactive GDP-bound form and an active GTP-bound form that is able to recruit to membranes different set of downstream effectors directly responsible for vesicle formation, movement, tethering and fusion. That Rab regulates endocytic recycling. Acts as a major regulator of membrane delivery during cytokinesis. Together with MYO5B and RAB8A participates in epithelial cell polarization. Together with RAB3IP, RAB8A, the exocyst complex, PARD3, PRKCI, ANXA2, CDC42 and DNMBP promotes transcytosis of PODXL to the apical membrane initiation sites (AMIS), apical surface formation and lumenogenesis. Together with MYO5B participates in CFTR trafficking to the plasma membrane and TF (Transferrin) recycling in nonpolarized cells. Required in a complex with MYO5B and RAB11FIP2 for the transport of NPC1L1 to the plasma membrane. Participates in the sorting and basolateral transport of CDH1 from the Golgi apparatus to the plasma membrane. Regulates the recycling of FCGRT (receptor of Fc region of monomeric Ig G) to basolateral membranes. May also play a role in melanosome transport and release from melanocytes. [UniProt]
Cellular Localization	Cell membrane; Lipid-anchor. Recycling endosome membrane; Lipid-anchor. Cleavage furrow. Cytoplasmic vesicle, phagosome. Note=Translocates with RAB11FIP2 from the vesicles of the endocytic recycling compartment to the plasma membrane. Localizes to the cleavage furrow. Colocalizes with PARD3, PRKCI, EXOC5, OCLN, PODXL and RAB8A in apical membrane initiation sites during the generation of apical surface and lumenogenesis. Recruited to phagosomes containing S.aureus or M.tuberculosis. [UniProt]
Calculated MW	24 kDa

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