ARG64474
anti-Pyruvate Carboxylase antibody
anti-Pyruvate Carboxylase antibody for Flow cytometry,IHC-Formalin-fixed paraffin-embedded sections and Human
Cancer antibody; Metabolism antibody; Signaling Transduction antibody
Overview
| Product Description | Goat Polyclonal antibody recognizes Pyruvate Carboxylase |
|---|---|
| Tested Reactivity | Hu |
| Predict Reactivity | Ms, Rat, Cow, Dog, Pig |
| Tested Application | FACS, IHC-P |
| Specificity | All reported variants (NP_000911.2; NP_001035806.1; NP_071504.2) represent identical protein. |
| Host | Goat |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | Pyruvate Carboxylase |
| Antigen Species | Human |
| Immunogen | C-KFKEVKKAYVEANQ |
| Conjugation | Un-conjugated |
| Alternate Names | PCB; Pyruvic carboxylase; EC 6.4.1.1; Pyruvate carboxylase, mitochondrial |
Application Instructions
| Application Suggestion |
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|---|---|---|---|---|---|---|---|
| Application Note | IHC-P: Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purified from goat serum by antigen affinity chromatography. |
| Buffer | Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA. |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 0.5% BSA |
| Concentration | 0.5 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # P11498 Human Pyruvate carboxylase, mitochondrial |
|---|---|
| Background | This gene encodes pyruvate carboxylase, which requires biotin and ATP to catalyse the carboxylation of pyruvate to oxaloacetate. The active enzyme is a homotetramer arranged in a tetrahedron which is located exclusively in the mitochondrial matrix. Pyruvate carboxylase is involved in gluconeogenesis, lipogenesis, insulin secretion and synthesis of the neurotransmitter glutamate. Mutations in this gene have been associated with pyruvate carboxylase deficiency. Alternatively spliced transcript variants with different 5' UTRs, but encoding the same protein, have been found for this gene. [provided by RefSeq, Jul 2008] |
| Research Area | Cancer antibody; Metabolism antibody; Signaling Transduction antibody |
| Calculated MW | 130 kDa |
Images (2) Click the Picture to Zoom In
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ARG64474 anti-Pyruvate Carboxylase antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human liver tissue. Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). The tissue section was stained with ARG64474 anti-Pyruvate Carboxylase antibody at 5 µg/ml dilution followed by AP-staining.
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ARG64474 anti-Pyruvate Carboxylase antibody FACS image
Flow Cytometry: Paraformaldehyde-fixed HeLa cells permeabilized with 0.5% Triton. Cells were stained with ARG64474 anti-Pyruvate Carboxylase antibody (blue line) at 10 µg/ml dilution for 1 hour, followed by incubation with Alexa Fluor® 488 labelled secondary antibody. IgG control: Unimmunized goat IgG (black line), followed by incubation with Alexa Fluor® 488 labelled secondary antibody.
