ARG43761

anti-Prominin 1 antibody [8B6]

anti-Prominin 1 antibody [8B6] for Flow cytometry,Western blot and Human,Mouse

Overview

Product Description Mouse Monoclonal antibody [8B6] recognizes Prominin 1
Tested Reactivity Hu, Ms
Tested Application FACS, WB
Host Mouse
Clonality Monoclonal
Clone 8B6
Isotype IgG2a
Target Name Prominin 1
Antigen Species Human
Immunogen Recombinant protein corresponding to a.a. P531 - H865 of Human Prominin-1
Conjugation Un-conjugated
Alternate Names CORD12; CD133; RP41; Antigen AC133; MCDR2; CD antigen CD133; PROML1; AC133; Prominin-like protein 1; Prominin-1; MSTP061; STGD4

Application Instructions

Application Suggestion
Tested Application Dilution
FACS1 - 3 µg/10^6 cells
WB1:500 - 1:2000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Observed Size 100~120 kDa

Properties

Form Liquid
Purification Affinity purification with immunogen.
Buffer 0.9% NaCl, 0.2% Na2HPO4 and 4% Trehalose
Stabilizer 4% Trehalose
Concentration 0.5 mg/ml
Storage Instruction For continuous use, store undiluted antibody [8B6] at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody [8B6] solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 19126 Mouse PROM1

GeneID: 8842 Human PROM1

Swiss-port # O43490 Human Prominin-1

Swiss-port # O54990 Mouse Prominin-1

Gene Symbol PROM1
Gene Full Name prominin 1
Background This gene encodes a pentaspan transmembrane glycoprotein. The protein localizes to membrane protrusions and is often expressed on adult stem cells, where it is thought to function in maintaining stem cell properties by suppressing differentiation. Mutations in this gene have been shown to result in retinitis pigmentosa and Stargardt disease. Expression of this gene is also associated with several types of cancer. This gene is expressed from at least five alternative promoters that are expressed in a tissue-dependent manner. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2009]
Function May play a role in cell differentiation, proliferation and apoptosis. Binds cholesterol in cholesterol-containing plasma membrane microdomains and may play a role in the organization of the apical plasma membrane in epithelial cells. During early retinal development acts as a key regulator of disk morphogenesis. Involved in regulation of MAPK and Akt signaling pathways. In neuroblastoma cells suppresses cell differentiation such as neurite outgrowth in a RET-dependent manner. [UniProt]
Calculated MW 97 kDa
PTM Isoform 1 and isoform 2 are glycosylated.
Acetylation at Lys-225, Lys-257 and Lys-264 by NAT8 and NAT8B may control PROM1 protein expression and its function in cell apoptosis.

Images (3) Click the Picture to Zoom In

  • ARG43761 anti-Prominin 1 antibody [8B6] WB image

    Western blot: CACO-2 and SW620 stained with ARG43761 anti-Prominin 1 antibody [8B6] at 0.5 μg/mL dilution.

  • ARG43761 anti-Prominin 1 antibody [8B6] FACS image

    Flow Cytometry: CACO-2 stained with ARG43761 anti-Prominin 1 antibody [8B6] at 1 μg / 10^6 cells dilution.

  • ARG43761 anti-Prominin 1 antibody [8B6] WB image

    Western blot: Mouse kidney stained with ARG43761 anti-Prominin 1 antibody [8B6] at 0.5 μg/mL dilution.