ARG11164
anti-Proinsulin antibody [CCI-17]
anti-Proinsulin antibody [CCI-17] for ELISA and Rat
Cell Biology and Cellular Response antibody; Metabolism antibody; Neuroscience antibody; Signaling Transduction antibody
Overview
| Product Description | Mouse Monoclonal antibody [CCI-17] recognizes Proinsulin |
|---|---|
| Tested Reactivity | Rat |
| Tested Application | ELISA |
| Specificity | This antibody binds specifically to proinsulin and does not bind to insulin or C-peptide. |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | CCI-17 |
| Isotype | IgG1 |
| Target Name | Proinsulin |
| Antigen Species | Rat |
| Immunogen | Immunized with fragment of rat proinsulin conjugated with a carrier protein. |
| Conjugation | Un-conjugated |
| Protein Full Name | Proinsulin |
| Alternate Names | IDDM; IDDM2; IDDM1; ILPR; MODY10; Insulin; IRDN |
Application Instructions
| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
|---|
Properties
| Form | Liquid |
|---|---|
| Purification | Protein A affinity purified. |
| Buffer | PBS (pH 7.4) and 0.1% Sodium azide |
| Preservative | 0.1% Sodium azide |
| Concentration | 1 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Gene Symbol | INS |
|---|---|
| Gene Full Name | insulin |
| Background | After removal of the precursor signal peptide, proinsulin is post-translationally cleaved into three peptides: the B chain and A chain peptides, which are covalently linked via two disulfide bonds to form insulin, and C-peptide. Binding of insulin to the insulin receptor (INSR) stimulates glucose uptake. A multitude of mutant alleles with phenotypic effects have been identified. There is a read-through gene, INS-IGF2, which overlaps with this gene at the 5' region and with the IGF2 gene at the 3' region. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jun 2010] |
| Function | Insulin decreases blood glucose concentration. It increases cell permeability to monosaccharides, amino acids and fatty acids. It accelerates glycolysis, the pentose phosphate cycle, and glycogen synthesis in liver. [UniProt] |
| Cellular Localization | Secreted |
| Research Area | Cell Biology and Cellular Response antibody; Metabolism antibody; Neuroscience antibody; Signaling Transduction antibody |
| Calculated MW | 12 kDa |
| PTM | Cleavage on pair of basic residues; Disulfide bond |
