ARG64376

anti-Proenkephalin antibody

anti-Proenkephalin antibody for IHC-Frozen sections,Western blot and Human

Neuroscience antibody

Overview

Product Description Goat Polyclonal antibody recognizes Proenkephalin
Tested Reactivity Hu
Tested Application IHC-Fr, WB
Host Goat
Clonality Polyclonal
Isotype IgG
Target Name Proenkephalin
Antigen Species Human
Immunogen C-RSHHQDGSDNEE
Conjugation Un-conjugated
Alternate Names 143-183; PENK-A; PE; 114-133; Proenkephalin-A; 237-258; OGF; Opioid growth factor

Application Instructions

Application Suggestion
Tested Application Dilution
IHC-Fr0.3 - 1 µg/ml
WB1 - 3 µg/ml
Application Note WB: Recommend incubate at RT for 1h.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Buffer Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA
Preservative 0.02% Sodium azide
Stabilizer 0.5% BSA
Concentration 0.5 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 5179 Human PENK

Swiss-port # P01210 Human Proenkephalin-A

Gene Symbol PENK
Gene Full Name proenkephalin
Background This gene encodes a preproprotein that is proteolytically processed to generate multiple protein products. These products include the pentapeptide opioids Met-enkephalin and Leu-enkephalin, which are stored in synaptic vesicles, then released into the synapse where they bind to mu- and delta-opioid receptors to modulate the perception of pain. Other non-opioid cleavage products may function in distinct biological activities. [provided by RefSeq, Jul 2015]
Function Met- and Leu-enkephalins compete with and mimic the effects of opiate drugs. They play a role in a number of physiologic functions, including pain perception and responses to stress. PENK(114-133) and PENK(237-258) increase glutamate release in the striatum. PENK(114-133) decreases GABA concentration in the striatum. [UniProt]
Research Area Neuroscience antibody
Calculated MW 31 kDa
PTM The N-terminal domain contains 6 conserved cysteines thought to be involved in disulfide bonding and/or processing.

Images (2) Click the Picture to Zoom In

  • ARG64376 anti-Proenkephalin antibody WB image

    Western Blot: Human Adrenal Gland lysate (35 µg protein in RIPA buffer) stained with ARG64376 anti-Proenkephalin antibody at 0.3 µg/ml dilution.

  • ARG64376 anti-Proenkephalin antibody IHC-Fr image

    Immunohistochemistry: PFA-perfused cryosection of Human hypothalamus tissue stained with ARG64376 anti-Proenkephalin antibody at 0.3 µg/ml dilution.