ARG64376
anti-Proenkephalin antibody
anti-Proenkephalin antibody for IHC-Frozen sections,Western blot and Human
Neuroscience antibody
Overview
| Product Description | Goat Polyclonal antibody recognizes Proenkephalin |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | IHC-Fr, WB |
| Host | Goat |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | Proenkephalin |
| Antigen Species | Human |
| Immunogen | C-RSHHQDGSDNEE |
| Conjugation | Un-conjugated |
| Alternate Names | 143-183; PENK-A; PE; 114-133; Proenkephalin-A; 237-258; OGF; Opioid growth factor |
Application Instructions
| Application Suggestion |
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|---|---|---|---|---|---|---|---|
| Application Note | WB: Recommend incubate at RT for 1h. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Buffer | Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 0.5% BSA |
| Concentration | 0.5 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | PENK |
| Gene Full Name | proenkephalin |
| Background | This gene encodes a preproprotein that is proteolytically processed to generate multiple protein products. These products include the pentapeptide opioids Met-enkephalin and Leu-enkephalin, which are stored in synaptic vesicles, then released into the synapse where they bind to mu- and delta-opioid receptors to modulate the perception of pain. Other non-opioid cleavage products may function in distinct biological activities. [provided by RefSeq, Jul 2015] |
| Function | Met- and Leu-enkephalins compete with and mimic the effects of opiate drugs. They play a role in a number of physiologic functions, including pain perception and responses to stress. PENK(114-133) and PENK(237-258) increase glutamate release in the striatum. PENK(114-133) decreases GABA concentration in the striatum. [UniProt] |
| Research Area | Neuroscience antibody |
| Calculated MW | 31 kDa |
| PTM | The N-terminal domain contains 6 conserved cysteines thought to be involved in disulfide bonding and/or processing. |
Images (2) Click the Picture to Zoom In
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ARG64376 anti-Proenkephalin antibody WB image
Western Blot: Human Adrenal Gland lysate (35 µg protein in RIPA buffer) stained with ARG64376 anti-Proenkephalin antibody at 0.3 µg/ml dilution.
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ARG64376 anti-Proenkephalin antibody IHC-Fr image
Immunohistochemistry: PFA-perfused cryosection of Human hypothalamus tissue stained with ARG64376 anti-Proenkephalin antibody at 0.3 µg/ml dilution.
