ARG23771
anti-Plexin A1 antibody
anti-Plexin A1 antibody for ELISA,ICC/IF,IHC-Frozen sections,IHC-Formalin-fixed paraffin-embedded sections,Immunoprecipitation,Western blot and Human,Mouse,Rat
Overview
| Product Description | Rabbit Polyclonal antibody recognizes Plexin A1 |
|---|---|
| Tested Reactivity | Hu, Ms, Rat |
| Tested Application | ELISA, ICC/IF, IHC-Fr, IHC-P, IP, WB |
| Specificity | This antibody detects a ~220 kDa protein corresponding to the apparent molecular mass of Plexin-A1 on SDS-PAGE immunoblots of neonatal rat brain and Cos-7 cells transfected with mouse Plexin-A1. In immunocytochemistry, this antibody co-localizes with anti-Myc in Cos-7 cells transfected with Myc-tagged mouse Plexin-A1. |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | Plexin A1 |
| Antigen Species | Human |
| Immunogen | Synthetic peptide (coupled to carrier protein) around the semaphorin domain (N-terminal half) of Human Plexin A1. This sequence is highly conserved in Rat and Mouse Plexin A1, and has low homology to other Plexin family members. |
| Conjugation | Un-conjugated |
| Alternate Names | Semaphorin receptor NOV; PLEXIN-A1; PLXN1; NOV; Plexin-A1; NOVP |
Application Instructions
| Application Suggestion |
|
||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Application Note | WB: Antibody is suggested to be diluted in 5% skimmed milk/Tris buffer with 0.04% Tween20 and incubated for 1 hour at room temperature. IHC-P: Antigen Retrieval: Boil tissue section in Tris/EDTA buffer (pH 9.0). * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
||||||||||||||
| Observed Size | 220 kDa |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purification with immunogen. |
| Buffer | PBS, 0.05% Sodium azide, 50% Glycerol and 1 mg/ml BSA. |
| Preservative | 0.05% Sodium azide |
| Stabilizer | 50% Glycerol and 1 mg/ml BSA |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | PLXNA1 |
| Gene Full Name | plexin A1 |
| Function | Coreceptor for SEMA3A, SEMA3C, SEMA3F and SEMA6D. Necessary for signaling by class 3 semaphorins and subsequent remodeling of the cytoskeleton. Plays a role in axon guidance, invasive growth and cell migration. Class 3 semaphorins bind to a complex composed of a neuropilin and a plexin. The plexin modulates the affinity of the complex for specific semaphorins, and its cytoplasmic domain is required for the activation of down-stream signaling events in the cytoplasm (By similarity). [UniProt] |
| Calculated MW | 211 kDa |
Images (3) Click the Picture to Zoom In
-
ARG23771 anti-Plexin A1 antibody ICC/IF image
Immunofluorescence: Double labeling using ARG23771 anti-Plexin A1 antibody in COS7 cells mock transfected (A,D) or transfected with Myc-tagged Mouse Plexin-A1 construct (B,E). The specificity of the binding in E was demonstrated by using Plexin A1 peptide in the presence of primary antibodies (C,F).
-
ARG23771 anti-Plexin A1 antibody WB image
Western blot: COS7 cells transfected with mouse Myc-tagged Plexin A1 and neonatal Rat brain (from left to right) stained with ARG23771 anti-Plexin A1 antibody.
-
ARG23771 anti-Plexin A1 antibody IP image
Immunoprecipitation: Myc-Plexin A1 transfected COS7 immunoprecipitated by ARG23771 anti-Plexin A1 antibody and stained with anti-Myc antibody.
