ARG45426

anti-Phospholipase D2 antibody

anti-Phospholipase D2 antibody for Flow cytometry,ICC/IF,Western blot and Human,Mouse,Rat

Overview

Product Description Rabbit Polyclonal antibody recognizes Phospholipase D2
Tested Reactivity Hu, Ms, Rat
Tested Application FACS, ICC/IF, WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name Phospholipase D2
Antigen Species Human
Immunogen Recombinant protein containing to human Phospholipase D2.
Conjugation Un-conjugated
Alternate Names PLD2; Choline phosphatase 2; PLD1C; PLD 2; Phosphatidylcholine-hydrolyzing phospholipase D2; Phospholipase D2; EC 3.1.4.4; hPLD2

Application Instructions

Application Suggestion
Tested Application Dilution
FACS1 - 3 µg/10^6 cells
ICC/IF2 μg/ml
WB0.25-0.5 μg/ml
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Observed Size 106 kDa

Properties

Form Liquid
Purification Affinity purified
Buffer 0.2% Na2HPO4, 0.9% NaCl, 0.05% Sodium azide and 4% Trehalose.
Preservative 0.05% Sodium azide
Stabilizer 4% Trehalose
Concentration 0.5 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 18806 Mouse PLD2

GeneID: 25097 Rat PLD2

GeneID: 5338 Human PLD2

Gene Symbol PLD2
Gene Full Name phospholipase D2
Background The protein encoded by this gene catalyzes the hydrolysis of phosphatidylcholine to phosphatidic acid and choline. The activity of the encoded enzyme is enhanced by phosphatidylinositol 4,5-bisphosphate and ADP-ribosylation factor-1. This protein localizes to the peripheral membrane and may be involved in cytoskeletal organization, cell cycle control, transcriptional regulation, and/or regulated secretion. Two transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Jul 2011]
Function May have a role in signal-induced cytoskeletal regulation and/or endocytosis. [UniProt]. [UniProt]
Cellular Localization Membrane; Peripheral membrane protein. [UniProt]. [UniProt]
Calculated MW 106 kDa
PTM Phosphorylated by FGR. [UniProt]. [UniProt]

Images (5) Click the Picture to Zoom In

  • ARG45426 anti-Phospholipase D2 antibody ICC/IF image

    Immunofluorescence: Hela stained with ARG45426 anti-Phospholipase D2 antibody at 2 μg/ml dilution.

  • ARG45426 anti-Phospholipase D2 antibody WB image

    Western blot: A375 stained with ARG45426 anti-Phospholipase D2 antibody at 0.5 μg/ml dilution.

  • ARG45426 anti-Phospholipase D2 antibody FACS image

    Flow Cytometry: U937 stained with ARG45426 anti-Phospholipase D2 antibody at 1 µg/10^6 cells dilution.

  • ARG45426 anti-Phospholipase D2 antibody WB image

    Western blot: Rat brain stained with ARG45426 anti-Phospholipase D2 antibody at 0.5 μg/ml dilution.

  • ARG45426 anti-Phospholipase D2 antibody WB image

    Western blot: Mouse lung stained with ARG45426 anti-Phospholipase D2 antibody at 0.5 μg/ml dilution.