ARG59033

anti-Phospholipase D1 antibody

anti-Phospholipase D1 antibody for Western blot and Human,Mouse

Overview

Product Description Rabbit Polyclonal antibody recognizes Phospholipase D1
Tested Reactivity Hu, Ms
Tested Application WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name Phospholipase D1
Antigen Species Human
Immunogen Recombinant protein corresponding to M1-H330 of Human Phospholipase D1.
Conjugation Un-conjugated
Alternate Names Choline phosphatase 1; PLD 1; Phosphatidylcholine-hydrolyzing phospholipase D1; Phospholipase D1; hPLD1; EC 3.1.4.4

Application Instructions

Application Suggestion
Tested Application Dilution
WB0.1 - 0.5 µg/ml
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Affinity purification with immunogen.
Buffer 0.2% Na2HPO4, 0.9% NaCl, 0.05% Sodium azide and 5% BSA.
Preservative 0.05% Sodium azide
Stabilizer 5% BSA
Concentration 0.5 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 5337 Human PLD1

Swiss-port # Q13393 Human Phospholipase D1

Gene Symbol PLD1
Gene Full Name phospholipase D1, phosphatidylcholine-specific
Background This gene encodes a phosphatidylcholine-specific phospholipase which catalyzes the hydrolysis of phosphatidylcholine in order to yield phosphatidic acid and choline. The enzyme may play a role in signal transduction and subcellular trafficking. Alternative splicing results in multiple transcript variants with both catalytic and regulatory properties. [provided by RefSeq, Sep 2011]
Function Implicated as a critical step in numerous cellular pathways, including signal transduction, membrane trafficking, and the regulation of mitosis. May be involved in the regulation of perinuclear intravesicular membrane traffic (By similarity). [UniProt]
Cellular Localization Cytoplasm, perinuclear region. Endoplasmic reticulum membrane; Lipid-anchor; Cytoplasmic side. Golgi apparatus membrane; Lipid-anchor; Cytoplasmic side. Late endosome membrane; Lipid- anchor; Cytoplasmic side. [UniProt]
Calculated MW 124 kDa

Images (1) Click the Picture to Zoom In

  • ARG59033 anti-Phospholipase D1 antibody WB image

    Western blot: 50 µg of Mouse cardiac muscle, 40 µg of 22RV1 and 40 µg of HeLa lysates stained with ARG59033 anti-Phospholipase D1 antibody at 0.5 µg/ml dilution.