ARG59455
anti-Peroxiredoxin 6 antibody
anti-Peroxiredoxin 6 antibody for Immunocytochemistry,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat
Overview
| Product Description | Rabbit Polyclonal antibody recognizes Peroxiredoxin 6 |
|---|---|
| Tested Reactivity | Hu, Ms, Rat |
| Tested Application | ICC, IHC-P, WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | Peroxiredoxin 6 |
| Antigen Species | Human |
| Immunogen | Recombinant protein corresponding to E15-P224 of Human Peroxiredoxin 6. |
| Conjugation | Un-conjugated |
| Alternate Names | 1-Cys; NSGPx; EC 3.1.1.-; Antioxidant protein 2; Non-selenium glutathione peroxidase; 24 kDa protein; Acidic calcium-independent phospholipase A2; AOP2; 1-Cys peroxiredoxin; aiPLA2; Peroxiredoxin-6; Liver 2D page spot 40; 1-Cys PRX; EC 1.11.1.15; EC 1.11.1.9; Red blood cells page spot 12; p29; PRX; HEL-S-128m |
Application Instructions
| Application Suggestion |
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| Application Note | IHC-P: Antigen Retrieval: Heat mediation was performed in Citrate buffer (pH 6.0) for 20 min. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purification with immunogen. |
| Buffer | 0.9% NaCl, 0.2% Na2HPO4, 0.05% Sodium azide and 5% BSA. |
| Preservative | 0.05% Sodium azide |
| Stabilizer | 5% BSA |
| Concentration | 0.5 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | PRDX6 |
| Gene Full Name | peroxiredoxin 6 |
| Background | The protein encoded by this gene is a member of the thiol-specific antioxidant protein family. This protein is a bifunctional enzyme with two distinct active sites. It is involved in redox regulation of the cell; it can reduce H(2)O(2) and short chain organic, fatty acid, and phospholipid hydroperoxides. It may play a role in the regulation of phospholipid turnover as well as in protection against oxidative injury. [provided by RefSeq, Jul 2008] |
| Function | Involved in redox regulation of the cell. Can reduce H(2)O(2) and short chain organic, fatty acid, and phospholipid hydroperoxides. May play a role in the regulation of phospholipid turnover as well as in protection against oxidative injury. [UniProt] |
| Cellular Localization | Cytoplasm. Lysosome. Note=Also found in lung secretory organelles (lamellar bodies). [UniProt] |
| Calculated MW | 25 kDa |
Images (8) Click the Picture to Zoom In
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ARG59455 anti-Peroxiredoxin 6 antibody ICC image
Immunocytochemistry: PC-3 cells were blocked with 10% goat serum and then stained with ARG59455 anti-Peroxiredoxin 6 antibody at 1 µg/ml dilution, overnight at 4°C.
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ARG59455 anti-Peroxiredoxin 6 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human mammary cancer stained with ARG59455 anti-Peroxiredoxin 6 antibody.
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ARG59455 anti-Peroxiredoxin 6 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human gastric cancer tissue. Antigen Retrieval: Heat mediation was performed in Citrate buffer (pH 6.0) for 20 min. The tissue section was blocked with 10% goat serum. The tissue section was then stained with ARG59455 anti-Peroxiredoxin 6 antibody at 1 µg/ml dilution, overnight at 4°C.
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ARG59455 anti-Peroxiredoxin 6 antibody WB image
Western blot: 50 µg of Mouse liver and 40 µg of 293T whole cell lysate stained with ARG59455 anti-Peroxiredoxin 6 antibody at 0.5 µg/ml dilution.
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ARG59455 anti-Peroxiredoxin 6 antibody WB image
Western blot: 50 µg of sample under reducing conditions. Human placenta, PC-3, K562, HeLa, Rat lung, Rat liver, Mouse lung and Mouse liver lysates stained with ARG59455 anti-Peroxiredoxin 6 antibody at 0.5 µg/ml dilution, overnight at 4°C.
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ARG59455 anti-Peroxiredoxin 6 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human Lung cancer tissue. Antigen Retrieval: Heat mediation was performed in Citrate buffer (pH 6.0) for 20 min. The tissue section was blocked with 10% goat serum. The tissue section was then stained with ARG59455 anti-Peroxiredoxin 6 antibody at 1 µg/ml dilution, overnight at 4°C.
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ARG59455 anti-Peroxiredoxin 6 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human ovarian cancer tissue. Antigen Retrieval: Heat mediation was performed in Citrate buffer (pH 6.0) for 20 min. The tissue section was blocked with 10% goat serum. The tissue section was then stained with ARG59455 anti-Peroxiredoxin 6 antibody at 1 µg/ml dilution, overnight at 4°C.
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ARG59455 anti-Peroxiredoxin 6 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Rat brain tissue. Antigen Retrieval: Heat mediation was performed in Citrate buffer (pH 6.0) for 20 min. The tissue section was blocked with 10% goat serum. The tissue section was then stained with ARG59455 anti-Peroxiredoxin 6 antibody at 1 µg/ml dilution, overnight at 4°C.
