ARG57048
anti-Peroxiredoxin 6 antibody [22E7]
anti-Peroxiredoxin 6 antibody [22E7] for Flow cytometry,ICC/IF,Western blot and Human
Overview
| Product Description | Mouse Monoclonal antibody [22E7] recognizes Peroxiredoxin 6 |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | FACS, ICC/IF, WB |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | 22E7 |
| Isotype | IgG2a, kappa |
| Target Name | Peroxiredoxin 6 |
| Antigen Species | Human |
| Immunogen | Recombinant fragment around aa. 1-224 of Human Peroxiredoxin 6. |
| Conjugation | Un-conjugated |
| Alternate Names | 1-Cys; NSGPx; EC 3.1.1.-; Antioxidant protein 2; Non-selenium glutathione peroxidase; 24 kDa protein; Acidic calcium-independent phospholipase A2; AOP2; 1-Cys peroxiredoxin; aiPLA2; Peroxiredoxin-6; Liver 2D page spot 40; 1-Cys PRX; EC 1.11.1.15; EC 1.11.1.9; Red blood cells page spot 12; p29; PRX; HEL-S-128m |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purification with Protein A. |
| Buffer | PBS (pH 7.4), 0.02% Sodium azide and 10% Glycerol. |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 10% Glycerol |
| Concentration | 1 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | PRDX6 |
| Gene Full Name | peroxiredoxin 6 |
| Background | The protein encoded by this gene is a member of the thiol-specific antioxidant protein family. This protein is a bifunctional enzyme with two distinct active sites. It is involved in redox regulation of the cell; it can reduce H(2)O(2) and short chain organic, fatty acid, and phospholipid hydroperoxides. It may play a role in the regulation of phospholipid turnover as well as in protection against oxidative injury. [provided by RefSeq, Jul 2008] |
| Function | Involved in redox regulation of the cell. Can reduce H(2)O(2) and short chain organic, fatty acid, and phospholipid hydroperoxides. May play a role in the regulation of phospholipid turnover as well as in protection against oxidative injury. [UniProt] |
| Calculated MW | 25 kDa |
Images (3) Click the Picture to Zoom In
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ARG57048 anti-Peroxiredoxin 6 antibody [22E7] ICC/IF image
Immunoflorescense: HeLa cell line stained with ARG57048 anti-Peroxiredoxin 6 antibody [22E7] at 1:100 (Green).
DAPI (Blue) for nucleus staining.
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ARG57048 anti-Peroxiredoxin 6 antibody [22E7] FACS image
Flow Cytometry: HeLa cells stained with ARG57048 anti-Peroxiredoxin 6 antibody [22E7] at 2-5 µg / 10^6 cells (red). Secondary antibody: Goat anti-Mouse IgG Alexa fluor 488 conjugate. Mouse monoclonal IgG was used as the isotype control (dark gray), cells without incubation with primary and secondary antibody was used as the negative control (light gray).
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ARG57048 anti-Peroxiredoxin 6 antibody [22E7] WB image
Western blot: 1) 50 ng of Recombinant PRDX6 protein, 40 µg of 2) HeLa cell lysate, 3) 293T cell lysate, 4) HepG2 cell lysate, 5) U87MG cell lysate, and 6) Ramos cell lysate stained with ARG57048 anti-Peroxiredoxin 6 antibody [22E7] at 1:1000 dilution.
