ARG59456
anti-Peroxiredoxin 5 antibody
anti-Peroxiredoxin 5 antibody for Flow cytometry,ICC/IF,IHC-Formalin-fixed paraffin-embedded sections and Human,Mouse,Rat
Overview
| Product Description | Rabbit Polyclonal antibody recognizes Peroxiredoxin 5 |
|---|---|
| Tested Reactivity | Hu, Ms, Rat |
| Tested Application | FACS, ICC/IF, IHC-P |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | Peroxiredoxin 5 |
| Antigen Species | Human |
| Immunogen | Recombinant protein corresponding to E66-D198 of Human Peroxiredoxin 5. |
| Conjugation | Un-conjugated |
| Alternate Names | PMP20; EC 1.11.1.15; Thioredoxin peroxidase PMP20; ACR1; Prx-V; PLP; PRXV; PRDX6; HEL-S-55; B166; Thioredoxin reductase; Peroxiredoxin-5, mitochondrial; AOEB166; Peroxiredoxin V; SBBI10; prx-V; Antioxidant enzyme B166; Alu corepressor 1; TPx type VI; Peroxisomal antioxidant enzyme; Liver tissue 2D-page spot 71B |
Application Instructions
| Application Suggestion |
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| Application Note | IHC-P: Antigen Retrieval: By heat mediation. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purification with immunogen. |
| Buffer | 0.9% NaCl, 0.2% Na2HPO4, 0.05% Sodium azide and 5% BSA. |
| Preservative | 0.05% Sodium azide |
| Stabilizer | 5% BSA |
| Concentration | 0.5 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | PRDX5 |
| Gene Full Name | peroxiredoxin 5 |
| Background | This gene encodes a member of the peroxiredoxin family of antioxidant enzymes, which reduce hydrogen peroxide and alkyl hydroperoxides. The encoded protein may play an antioxidant protective role in different tissues under normal conditions and during inflammatory processes. This protein interacts with peroxisome receptor 1. The crystal structure of this protein in its reduced form has been resolved to 1.5 angstrom resolution. This gene uses alternate in-frame translation initiation sites to generate mitochondrial or peroxisomal/cytoplasmic forms. Three transcript variants encoding distinct isoforms have been identified for this gene. [provided by RefSeq, Jul 2008] |
| Function | Reduces hydrogen peroxide and alkyl hydroperoxides with reducing equivalents provided through the thioredoxin system. Involved in intracellular redox signaling. [UniProt] |
| Cellular Localization | Isoform Mitochondrial: Mitochondrion. Isoform Cytoplasmic+peroxisomal: Cytoplasm. Peroxisome matrix. Note=Imported into peroxisomes via peroxisomal targeting signal 1 receptor PEX5. [UniProt] |
| Calculated MW | 22 kDa |
Images (6) Click the Picture to Zoom In
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ARG59456 anti-Peroxiredoxin 5 antibody ICC image
Immunocytochemistry: SMMC-7721 cells stained with ARG59456 anti-Peroxiredoxin 5 antibody.
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ARG59456 anti-Peroxiredoxin 5 antibody ICC/IF image
Immunofluorescence: HeLa cells were blocked with 10% goat serum and then stained with ARG59456 anti-Peroxiredoxin 5 antibody (green) at 2 µg/ml dilution, overnight at 4°C. DAPI (blue) for nuclear staining.
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ARG59456 anti-Peroxiredoxin 5 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Mouse intestine stained with ARG59456 anti-Peroxiredoxin 5 antibody.
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ARG59456 anti-Peroxiredoxin 5 antibody FACS image
Flow Cytometry: A549 cells were blocked with 10% normal goat serum and then stained with ARG59456 anti-Peroxiredoxin 5 antibody (blue) at 1 µg/10^6 cells for 30 min at 20°C, followed by incubation with DyLight®488 labelled secondary antibody. Isotype control antibody (green) was rabbit IgG (1 µg/10^6 cells) used under the same conditions. Unlabelled sample (red) was also used as a control.
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ARG59456 anti-Peroxiredoxin 5 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Rat intestine stained with ARG59456 anti-Peroxiredoxin 5 antibody.
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ARG59456 anti-Peroxiredoxin 5 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human lung cancer stained with ARG59456 anti-Peroxiredoxin 5 antibody.
