ARG57055
anti-Peroxiredoxin 5 antibody [6A10]
anti-Peroxiredoxin 5 antibody [6A10] for Flow cytometry,Western blot and Human
Overview
| Product Description | Mouse Monoclonal antibody [6A10] recognizes Peroxiredoxin 5 |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | FACS, WB |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | 6A10 |
| Isotype | IgG1, kappa |
| Target Name | Peroxiredoxin 5 |
| Antigen Species | Human |
| Immunogen | Recombinant fragment around aa. 53-214 of Human Peroxiredoxin 5. |
| Conjugation | Un-conjugated |
| Alternate Names | PMP20; EC 1.11.1.15; Thioredoxin peroxidase PMP20; ACR1; Prx-V; PLP; PRXV; PRDX6; HEL-S-55; B166; Thioredoxin reductase; Peroxiredoxin-5, mitochondrial; AOEB166; Peroxiredoxin V; SBBI10; prx-V; Antioxidant enzyme B166; Alu corepressor 1; TPx type VI; Peroxisomal antioxidant enzyme; Liver tissue 2D-page spot 71B |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purification with Protein A. |
| Buffer | PBS (pH 7.4), 0.02% Sodium azide and 10% Glycerol. |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 10% Glycerol |
| Concentration | 1 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | PRDX5 |
| Gene Full Name | peroxiredoxin 5 |
| Background | This gene encodes a member of the peroxiredoxin family of antioxidant enzymes, which reduce hydrogen peroxide and alkyl hydroperoxides. The encoded protein may play an antioxidant protective role in different tissues under normal conditions and during inflammatory processes. This protein interacts with peroxisome receptor 1. The crystal structure of this protein in its reduced form has been resolved to 1.5 angstrom resolution. This gene uses alternate in-frame translation initiation sites to generate mitochondrial or peroxisomal/cytoplasmic forms. Three transcript variants encoding distinct isoforms have been identified for this gene. [provided by RefSeq, Jul 2008] |
| Function | Reduces hydrogen peroxide and alkyl hydroperoxides with reducing equivalents provided through the thioredoxin system. Involved in intracellular redox signaling. [UniProt] |
| Calculated MW | 22 kDa |
Images (2) Click the Picture to Zoom In
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ARG57055 anti-Peroxiredoxin 5 antibody [6A10] FACS image
Flow Cytometry: HeLa cell line stained with ARG57055 anti-Peroxiredoxin 5 antibody [6A10] at 2-5 µg for 1x10^6 cells (red line). Secondary antibody: Goat anti-Mouse IgG Alexa fluor 488 conjugate. Isotype control antibody was Mouse IgG (black line).
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ARG57055 anti-Peroxiredoxin 5 antibody [6A10] WB image
Western blot: 1) 50 ng of Recombinant PRDX5 protein, 40 µg of 2) MCF-7 cell lysate, 3) A549 cell lysate, 4) HeLa cell lysate, 5) 293T cell lysate, 6) Jurkat cell lysate stained with ARG57055 anti-Peroxiredoxin 5 antibody [6A10] at 1:1000.
