ARG52393
anti-Periostin (pan) antibody
anti-Periostin (pan) antibody for Immunohistochemistry,Western blot and Human,Mouse,Rat,Chicken
Cancer antibody; Cell Biology and Cellular Response antibody; Controls and Markers antibody; Signaling Transduction antibody
1
Overview
| Product Description | Rabbit Polyclonal antibody recognizes Periostin (pan) |
|---|---|
| Tested Reactivity | Hu, Ms, Rat, Chk |
| Predict Reactivity | Amph, Bird, Fsh, Mamm |
| Tested Application | IHC, WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | Periostin (pan) |
| Antigen Species | Mouse |
| Immunogen | Synthetic peptide corresponding to amino acid residues from the fasciclin domain 1 of mouse periostin |
| Conjugation | Un-conjugated |
| Alternate Names | OSF2; PN; Osteoblast-specific factor 2; Periostin; OSF-2; PDLPOSTN |
Application Instructions
| Application Suggestion |
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| Application Note | Specific for three periostin bands around ~93 kDa in mouse lung extract. The immunogenic peptide is present in all known splice variants of periostin and therefore this antibody recognizes all known molecular forms of periostin. The antibody also works well for immunohistochemistry on paraformaldehyde-fixed sections with a simple antigenretrieval protocol (incubate slides for 20 minutes at 90º C in 10 mM sodium citRate (pH 6.0)/ 0.1 % Tween-20). * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity Purified |
| Buffer | PBS |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | POSTN |
| Gene Full Name | periostin, osteoblast specific factor |
| Background | Periostin is a matricellular protein, i.e. an extracellular matrix protein that interacts both with other ECM proteins and with cell-surface receptors. Like many other matricellular proteins, the function of periostin is important both in embryonic development and in the remodeling of adult tissues in response to pathological insults. Periostin was originally isolated as an osteoblast-specific marker that functions as a cell adhesion molecule for preosteoblasts and is thought to be involved in osteoblast recruitment, attachment and spreading (Kruzynska-Frejtag A. et al., 2004). Periostin has recently been shown to promote collagen fibrogenesis, inhibit differentiation of progenitor cells into cardiomyocytes and to be essential in maintaining the biomechanical properties of the adult myocardium (Norris et al., 2008) |
| Research Area | Cancer antibody; Cell Biology and Cellular Response antibody; Controls and Markers antibody; Signaling Transduction antibody |
| Calculated MW | 93 kDa |
| PTM | Gamma-carboxylation is controversial. Gamma-carboxyglutamated; gamma-carboxyglutamate residues are formed by vitamin K dependent carboxylation; this may be required for calcium binding (PubMed:18450759). According to a more recent report, does not contain vitamin K-dependent gamma-carboxyglutamate residues (PubMed:26273833). |
Images (1) Click the Picture to Zoom In
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ARG52393 anti-Periostin (pan) antibody WB image
Western blot: rat lung lysate stained with ARG52393 anti-Periostin (pan) antibody showing specific immunolabeling of the ~ 93 kDa periostin protein triplet.
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Review for anti-Periostin (pan) antibody
Application：WB
Sample：Rat brain
Sample Loading Amount：30 µg
Primary Antibody Dilution Factor：1:1000
Primary Antibody Incubation Time：overnight
Primary Antibody Incubation Temperature：4 ºC
