ARG63603
anti-Pericentrin 1 / NUP85 antibody
anti-Pericentrin 1 / NUP85 antibody for Immunoprecipitation,Western blot and Human
Controls and Markers antibody
Overview
| Product Description | Goat Polyclonal antibody recognizes Pericentrin 1 / NUP85 |
|---|---|
| Tested Reactivity | Hu |
| Predict Reactivity | Ms, Rat |
| Tested Application | IP, WB |
| Specificity | Please note that, in contrast to other anti-Pericentrin antibodies available, this ab is NOT expected to recognize Kendrin (also known as Pericentrin 2). This may explain why it recognizes a 75KDa band (the expected size according to NP_079120) and not a 220kDa band (as reported for other anti-pericentrin antibodies). |
| Host | Goat |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | Pericentrin 1 / NUP85 |
| Antigen Species | Human |
| Immunogen | C-ARAIIREGSLEGS |
| Conjugation | Un-conjugated |
| Alternate Names | Nup75; FROUNT; Nucleoporin Nup85; Nucleoporin Nup75; Nuclear pore complex protein Nup85; Pericentrin-1; 85 kDa nucleoporin |
Application Instructions
| Application Suggestion |
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|---|---|---|---|---|---|---|---|
| Application Note | WB: Recommend incubate at RT for 1h. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Buffer | Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 0.5% BSA |
| Concentration | 0.5 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # Q9BW27 Human Nuclear pore complex protein Nup85 |
|---|---|
| Background | Bidirectional transport of macromolecules between the cytoplasm and nucleus occurs through nuclear pore complexes (NPCs) embedded in the nuclear envelope. NPCs are composed of subcomplexes, and NUP85 is part of one such subcomplex, Nup107-160. [provided by RefSeq, Jul 2008] |
| Research Area | Controls and Markers antibody |
| Calculated MW | 75 kDa |
Images (1) Click the Picture to Zoom In
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ARG63603 anti-Pericentrin 1 / NUP85 antibody WB image
Western Blot: HeLa lysate (35 µg protein in RIPA buffer) stained with ARG63603 anti-Pericentrin 1 / NUP85 antibody at 1 µg/ml dilution.
