ARG64324

anti-Patched 1 antibody

anti-Patched 1 antibody for ICC/IF and Zebrafish

Cancer antibody; Cell Biology and Cellular Response antibody; Developmental Biology antibody; Neuroscience antibody; Signaling Transduction antibody

Overview

Product Description Goat Polyclonal antibody recognizes Patched 1
Tested Reactivity Zfsh
Tested Application ICC/IF
Host Goat
Clonality Polyclonal
Isotype IgG
Target Name Patched 1
Antigen Species Zebrafish
Immunogen C-QTGSKKEPFNYSQ
Conjugation Un-conjugated
Alternate Names NBCCS; PTCH; HPE7; Protein patched homolog 1; PTC; PTCH11; BCNS; PTC1

Application Instructions

Application Suggestion
Tested Application Dilution
ICC/IF1 - 3 µg/ml
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Buffer Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA
Preservative 0.02% Sodium azide
Stabilizer 0.5% BSA
Concentration 0.5 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 30189 Zebrafish PTCH1

Gene Symbol ptch1
Gene Full Name patched 1
Background This gene encodes a member of the patched gene family. The encoded protein is the receptor for sonic hedgehog, a secreted molecule implicated in the formation of embryonic structures and in tumorigenesis, as well as the desert hedgehog and indian hedgehog proteins. This gene functions as a tumor suppressor. Mutations of this gene have been associated with basal cell nevus syndrome, esophageal squamous cell carcinoma, trichoepitheliomas, transitional cell carcinomas of the bladder, as well as holoprosencephaly. Alternative splicing results in multiple transcript variants encoding different isoforms. Additional splice variants have been described, but their full length sequences and biological validity cannot be determined currently. [provided by RefSeq, Jul 2008]
Research Area Cancer antibody; Cell Biology and Cellular Response antibody; Developmental Biology antibody; Neuroscience antibody; Signaling Transduction antibody
Calculated MW 161 kDa
PTM Glycosylation is necessary for SHH binding.
In the absence of Hh ligands, ubiquitination by ITCH at Lys-1426 promotes endocytosis and both proteasomal and lysosomal degradation.