ARG23249
anti-Pan B-Cells antibody [CVS36] (PE)
anti-Pan B-Cells antibody [CVS36] (PE) for Flow cytometry and Horse
Overview
| Product Description | PE-conjugated Mouse Monoclonal antibody [CVS36] recognizes Pan B-Cells Mouse anti Horse Pan B-Cells, clone CVS36 is a monoclonal antibody directed against equine Ig light chains. Characterization studies demonstrated that clone CVS36 bound 100% of CD5-ve peripheral blood lymphocytes and recognized all equine B-cells (Lunn et al. 1998) As the antigen recognized by clone CVS36 appears to be present on the surface of all equine B-cells it is therefore is a reagent that can be used as a pan B-cell marker for domestic horses (Breathnach et al. 2005). Specific anti equine reagents have yet to be fully characterized for the typically recognized B-cell makers such as CD19, CD20, CD21, CD22 and CD79. While testing has demonstrated the cross reactivity of some monoclonal B-cell markers raised against other species with equine B cells, such as the Mouse anti Human CD79a antibody, (clone HM57), an overview of which may be found in the report of the second equine leucocyte antigen workshop (Lunn et al. 1998). With specificity for equine Ig light chains, clone CVS36 may be used to detect all equine immunoglobulin classes and subclasses in ELISA applications (Lunn et al. 1998). |
|---|---|
| Tested Reactivity | Hrs |
| Tested Application | FACS |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | CVS36 |
| Isotype | IgG1 |
| Target Name | Pan B-Cells |
| Antigen Species | Horse |
| Immunogen | Purified Equine Ig. |
| Conjugation | PE |
Application Instructions
| Application Suggestion |
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|---|---|---|---|---|---|
| Application Note | FACS: Use 10 µl of the suggested working dilution to label 10^6 cells in 100 µl. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purification with Protein A. |
| Buffer | PBS, 0.09% Sodium azide, 1% BSA and 5% Sucrose. |
| Preservative | 0.09% Sodium azide |
| Stabilizer | 1% BSA and 5% Sucrose |
| Storage Instruction | Aliquot and store in the dark at 2-8°C. Keep protected from prolonged exposure to light. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Images (1) Click the Picture to Zoom In
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ARG23249 anti-Pan B-Cells antibody [CVS36] (PE) FACS image
Flow Cytometry: Horse peripheral blood lymphocytes stained with ARG23249 anti-Pan B-Cells antibody [CVS36] (PE).
