ARG64666

anti-PTGER3 / EP3 antibody

anti-PTGER3 / EP3 antibody for Western blot and Human

Metabolism antibody; Signaling Transduction antibody

Overview

Product Description Goat Polyclonal antibody recognizes PTGER3 / EP3
Tested Reactivity Hu
Tested Application WB
Specificity This antibody is expected to recognize isoforms 1, 2 and 3 (NP_000948.2, NP_942005.2 and NP_942006.1).
Host Goat
Clonality Polyclonal
Isotype IgG
Target Name PTGER3 / EP3
Antigen Species Human
Immunogen KLLREPCSVQLS
Conjugation Un-conjugated
Alternate Names PGE receptor EP3 subtype; PGE2 receptor EP3 subtype; EP3-IV; PGE2-R; EP3-VI; EP3e; EP3; Prostanoid EP3 receptor; EP3-III; Prostaglandin E2 receptor EP3 subtype; EP3-I; EP3-II

Application Instructions

Application Suggestion
Tested Application Dilution
WB1 - 3 µg/ml
Application Note WB: Recommend incubate at RT for 1h.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Buffer Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA
Preservative 0.02% Sodium azide
Stabilizer 0.5% BSA
Concentration 0.5 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 5733 Human PTGER3

Swiss-port # P43115 Human Prostaglandin E2 receptor EP3 subtype

Background The protein encoded by this gene is a member of the G-protein coupled receptor family. This protein is one of four receptors identified for prostaglandin E2 (PGE2). This receptor may have many biological functions, which involve digestion, nervous system, kidney reabsorption, and uterine contraction activities. Studies of the mouse counterpart suggest that this receptor may also mediate adrenocorticotropic hormone response as well as fever generation in response to exogenous and endogenous stimuli. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2009]
Research Area Metabolism antibody; Signaling Transduction antibody
Calculated MW 43 kDa

Images (1) Click the Picture to Zoom In

  • ARG64666 anti-PTGER3 / EP3 antibody WB image

    Western Blot: Human Adipose lysate (35 µg protein in RIPA buffer) stained with ARG64666 anti-PTGER3 / EP3 antibody at 1 µg/ml dilution.