ARG64229
anti-PTF1A / PFT1-P48 antibody
anti-PTF1A / PFT1-P48 antibody for Western blot and Human,Mouse
Gene Regulation antibody; Neuroscience antibody
Overview
| Product Description | Goat Polyclonal antibody recognizes PTF1A / PFT1-P48 |
|---|---|
| Tested Reactivity | Hu, Ms |
| Predict Reactivity | Dog, Rat, Zfsh |
| Tested Application | WB |
| Host | Goat |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | PTF1A / PFT1-P48 |
| Antigen Species | Human |
| Immunogen | C-WTDEKQLKEQN |
| Conjugation | Un-conjugated |
| Alternate Names | p48 DNA-binding subunit of transcription factor PTF1; bHLHa29; Pancreas transcription factor 1 subunit alpha; Pancreas-specific transcription factor 1a; Class A basic helix-loop-helix protein 29; PACA; bHLH transcription factor p48; PAGEN2; PTF1-p48 |
Application Instructions
| Application Suggestion |
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|---|---|---|---|---|---|
| Application Note | WB: Recommend incubate at RT for 1h. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Buffer | Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 0.5% BSA |
| Concentration | 0.5 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # Q7RTS3 Human Pancreas transcription factor 1 subunit alpha Swiss-port # Q9QX98 Mouse Pancreas transcription factor 1 subunit alpha |
|---|---|
| Background | This gene encodes a protein that is a component of the pancreas transcription factor 1 complex (PTF1) and is known to have a role in mammalian pancreatic development. The protein plays a role in determining whether cells allocated to the pancreatic buds continue towards pancreatic organogenesis or revert back to duodenal fates. The protein is thought to be involved in the maintenance of exocrine pancreas-specific gene expression including elastase 1 and amylase. Mutations in this gene cause cerebellar agenesis and loss of expression is seen in ductal type pancreas cancers. [provided by RefSeq, Jul 2008] |
| Research Area | Gene Regulation antibody; Neuroscience antibody |
| Calculated MW | 35 kDa |
Images (2) Click the Picture to Zoom In
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ARG64229 anti-PTF1A / PFT1-P48 antibody WB image
Western blot: 35 µg of U2OS (A), U2OS + peptide (B), Jurkat (C), Jurkat + peptide (D), NIH/3T3 (E), NIH/3T3 + peptide (F) cell lysates (in RIPA buffer) stained with ARG64229 anti-PTF1A / PFT1-P48 antibody at 1 µg/ml (A, B) and 0.5 µg/ml (C-F) dilutions and incubated at RT for 1 hour.
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ARG64229 anti-PTF1A / PFT1-P48 antibody WB image
Western blot: 35 µg of Human pancreas lysate (in RIPA buffer) stained with ARG64229 anti-PTF1A / PFT1-P48 antibody at 0.5 µg/ml dilution and incubated at RT for 1 hour.
