ARG63682
anti-PSMD2 / Proteasome 26S S2 antibody
anti-PSMD2 / Proteasome 26S S2 antibody for Flow cytometry,ICC/IF,Western blot and Human,Mouse,Rat
Cell Biology and Cellular Response antibody
Overview
| Product Description | Goat Polyclonal antibody recognizes PSMD2 / Proteasome 26S S2 |
|---|---|
| Tested Reactivity | Hu, Ms, Rat |
| Predict Reactivity | Cow, Dog |
| Tested Application | FACS, ICC/IF, WB |
| Host | Goat |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | PSMD2 / Proteasome 26S S2 |
| Antigen Species | Human |
| Immunogen | C-VILRKNPNYDL |
| Conjugation | Un-conjugated |
| Alternate Names | RPN1; TRAP2; 26S proteasome subunit p97; S2; Protein 55.11; P97; 26S proteasome non-ATPase regulatory subunit 2; 26S proteasome regulatory subunit S2; 26S proteasome regulatory subunit RPN1; Tumor necrosis factor type 1 receptor-associated protein 2 |
Application Instructions
| Application Suggestion |
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| Application Note | WB: Recommend incubate at RT for 1h. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Buffer | Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 0.5% BSA |
| Concentration | 0.5 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
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| Background | The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. The 20S core is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. The 19S regulator is composed of a base, which contains 6 ATPase subunits and 2 non-ATPase subunits, and a lid, which contains up to 10 non-ATPase subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes one of the non-ATPase subunits of the 19S regulator lid. In addition to participation in proteasome function, this subunit may also participate in the TNF signalling pathway since it interacts with the tumor necrosis factor type 1 receptor. A pseudogene has been identified on chromosome 1. [provided by RefSeq, Jul 2008] |
| Research Area | Cell Biology and Cellular Response antibody |
| Calculated MW | 100 kDa |
Images (3) Click the Picture to Zoom In
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ARG63682 anti-PSMD2 / Proteasome 26S S2 antibody ICC/IF image
Immunofluorescence: Paraformaldehyde fixed HeLa cells permeabilized with 0.15% Triton. Cells were stained with ARG63682 anti-PSMD2 / Proteasome 26S S2 antibody (green) at 10 µg/ml dilution for 1 hour. DAPI (blue) for nuclear staining. Negative control: Unimmunized goat IgG (green) at 10 µg/ml dilution.
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ARG63682 anti-PSMD2 / Proteasome 26S S2 antibody WB image
Western blot: 35 µg of HeLa (A), U251 (B), KNRK (C) and NIH/3T3 (D) cell lysates (in RIPA buffer) stained with ARG63682 anti-PSMD2 / Proteasome 26S S2 antibody at 1 µg/ml (A-C) and 0.3 µg/ml (D) dilutions and incubated at RT for 1 hour.
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ARG63682 anti-PSMD2 / Proteasome 26S S2 antibody FACS image
Flow Cytometry: Paraformaldehyde-fixed HeLa cells permeabilized with 0.5% Triton. Cells were stained with ARG63682 anti-PSMD2 / Proteasome 26S S2 antibody (blue line) at 10 µg/ml dilution for 1 hour, followed by incubation with Alexa FluorR 488 labelled secondary antibody. IgG control: Unimmunized goat IgG (black line).
