ARG64947
anti-PSMB8 / LMP7 antibody
anti-PSMB8 / LMP7 antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human
Cell Biology and Cellular Response antibody
Overview
| Product Description | Goat Polyclonal antibody recognizes PSMB8 / LMP7 |
|---|---|
| Tested Reactivity | Hu |
| Predict Reactivity | Ms, Rat, Cow, Dog, Pig |
| Tested Application | IHC-P, WB |
| Specificity | This antibody is expected to recognize both reported isoforms (NP_004150.1; NP_683720.2). |
| Host | Goat |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | PSMB8 / LMP7 |
| Antigen Species | Human |
| Immunogen | C-DVSDLLHQYREANQ |
| Conjugation | Un-conjugated |
| Alternate Names | Proteasome subunit beta-5i; Multicatalytic endopeptidase complex subunit C13; Proteasome subunit beta type-8; D6S216E; Really interesting new gene 10 protein; JMP; LMP7; NKJO; PSMB5i; Proteasome component C13; EC 3.4.25.1; RING10; Low molecular mass protein 7; ALDD; D6S216; Macropain subunit C13 |
Application Instructions
| Application Suggestion |
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|---|---|---|---|---|---|---|---|
| Application Note | WB: Recommend incubate at RT for 1h. IHC-P: Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Buffer | Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 0.5% BSA |
| Concentration | 0.5 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Background | The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a member of the proteasome B-type family, also known as the T1B family, that is a 20S core beta subunit. This gene is located in the class II region of the MHC (major histocompatibility complex). Expression of this gene is induced by gamma interferon and this gene product replaces catalytic subunit 3 (proteasome beta 5 subunit) in the immunoproteasome. Proteolytic processing is required to generate a mature subunit. Two alternative transcripts encoding two isoforms have been identified; both isoforms are processed to yield the same mature subunit. [provided by RefSeq, Jul 2008] |
| Research Area | Cell Biology and Cellular Response antibody |
| Calculated MW | 30 kDa |
| PTM | Autocleaved. The resulting N-terminal Thr residue of the mature subunit is responsible for the nucleophile proteolytic activity. |
Images (2) Click the Picture to Zoom In
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ARG64947 anti-PSMB8 / LMP7 antibody WB image
Western blot: Human Liver lysate (35 µg protein in RIPA buffer) stained with ARG64947 anti-PSMB8 / LMP7 antibody at 0.01 µg/ml dilution.
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ARG64947 anti-PSMB8 / LMP7 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human spleen tissue. Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). The tissue section was stained with ARG64947 anti-PSMB8 / LMP7 antibody at 3.75 µg/ml dilution followed by AP-staining.
