ARG64946
anti-PSMB10 antibody
anti-PSMB10 antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human
Cell Biology and Cellular Response antibody; Immune System antibody
Overview
| Product Description | Goat Polyclonal antibody recognizes PSMB10 |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | IHC-P, WB |
| Host | Goat |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | PSMB10 |
| Antigen Species | Human |
| Immunogen | C-PTEPVKRSGRYH |
| Conjugation | Un-conjugated |
| Alternate Names | LMP10; Proteasome MECl-1; Proteasome subunit beta-2i; Low molecular mass protein 10; Macropain subunit MECl-1; EC 3.4.25.1; Proteasome subunit beta type-10; MECL1; Multicatalytic endopeptidase complex subunit MECl-1; beta2i |
Application Instructions
| Application Suggestion |
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|---|---|---|---|---|---|---|---|
| Application Note | IHC-P: Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). WB: Recommend incubate at RT for 1h. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Buffer | Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 0.5% BSA |
| Concentration | 0.5 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Background | The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a member of the proteasome B-type family, also known as the T1B family, that is a 20S core beta subunit. Proteolytic processing is required to generate a mature subunit. Expression of this gene is induced by gamma interferon, and this gene product replaces catalytic subunit 2 (proteasome beta 7 subunit) in the immunoproteasome. [provided by RefSeq, Jul 2008] |
| Research Area | Cell Biology and Cellular Response antibody; Immune System antibody |
| Calculated MW | 29 kDa |
| PTM | Autocleaved. The resulting N-terminal Thr residue of the mature subunit is responsible for the nucleophile proteolytic activity. |
Images (2) Click the Picture to Zoom In
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ARG64946 anti-PSMB10 antibody WB image
Western blot: 35 µg of Human Lung lysate stained with ARG64946 anti-PSMB10 antibody at 0.3 µg/ml dilution.
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ARG64946 anti-PSMB10 antibody IHC image
Immunohistochemistry: paraffin-embedded Human Tonsil (Steamed antigen retrieval with citrate buffer pH 6) stained with ARG64946 anti-PSMB10 antibody at 5 µg/ml dilution, followed by AP-staining.
