ARG40957
anti-PSMA5 / Proteasome 19S S5A antibody
anti-PSMA5 / Proteasome 19S S5A antibody for ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat
Overview
| Product Description | Mouse Monoclonal antibody recognizes PSMA5 / Proteasome 19S S5A |
|---|---|
| Tested Reactivity | Hu, Ms, Rat |
| Predict Reactivity | Bov |
| Tested Application | ICC/IF, IHC-P, WB |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | 426CT8.5.1 |
| Isotype | IgG1 |
| Target Name | PSMA5 / Proteasome 19S S5A |
| Antigen Species | Human |
| Immunogen | Purified His-tagged PSMA5 protein (fragment). |
| Conjugation | Un-conjugated |
| Alternate Names | Proteasome subunit alpha type-5; Macropain zeta chain; PSC5; Multicatalytic endopeptidase complex zeta chain; EC 3.4.25.1; ZETA; Proteasome zeta chain |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||||
| Positive Control | K562 | ||||||||
| Observed Size | 26 kDa |
Properties
| Form | Liquid |
|---|---|
| Purification | Purification with Protein G. |
| Buffer | PBS and 0.09% (W/V) Sodium azide. |
| Preservative | 0.09% (W/V) Sodium azide |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | PSMA5 |
| Gene Full Name | proteasome subunit alpha 5 |
| Background | The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a member of the peptidase T1A family, that is a 20S core alpha subunit. Multiple alternatively spliced transcript variants encoding two distinct isoforms have been found for this gene. [provided by RefSeq, Dec 2010] |
| Function | The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity. [UniProt] |
| Cellular Localization | Cytoplasm. Nucleus. [UniProt] |
| Calculated MW | 26 kDa |
Images (3) Click the Picture to Zoom In
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ARG40957 anti-PSMA5 / Proteasome 19S S5A antibody ICC/IF image
Immunofluorescence: HeLa cells stained with ARG40957 anti-PSMA5 / Proteasome 19S S5A antibody (green) at 1:25 dilution. Cytoplasmic actin was counterstained with Alexa Fluor® 555 conjugated with Phalloidin (red).
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ARG40957 anti-PSMA5 / Proteasome 19S S5A antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human testis tissue stained with ARG40957 anti-PSMA5 / Proteasome 19S S5A antibody at 1:25 dilution.
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ARG40957 anti-PSMA5 / Proteasome 19S S5A antibody WB image
Western blot: 35 µg of K562 cell lysate stained with ARG40957 anti-PSMA5 / Proteasome 19S S5A antibody.
