ARG65301
anti-PSMA4 antibody
anti-PSMA4 antibody for Western blot and Human,Mouse,Rat
Cell Biology and Cellular Response antibody
Overview
| Product Description | Goat Polyclonal antibody recognizes PSMA4 |
|---|---|
| Tested Reactivity | Hu, Ms, Rat |
| Predict Reactivity | Dog |
| Tested Application | WB |
| Specificity | This antibody is expected to recognize both reported isoforms (NP_002780.1; NP_001096138.1). Reported variants represent identical protein: NP_002780.1, NP_001096137.1 |
| Host | Goat |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | PSMA4 |
| Antigen Species | Human |
| Immunogen | C-SDANVLTNELR |
| Conjugation | Un-conjugated |
| Alternate Names | Proteasome component C9; PSC9; Proteasome subunit alpha type-4; HC9; Proteasome subunit L; HsT17706; Multicatalytic endopeptidase complex subunit C9; Macropain subunit C9; EC 3.4.25.1 |
Application Instructions
| Application Suggestion |
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|---|---|---|---|---|---|
| Application Note | WB: Recommend incubate at RT for 1h. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Buffer | Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 0.5% BSA |
| Concentration | 0.5 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Background | The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a member of the peptidase T1A family, that is a 20S core alpha subunit. Three alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008] |
| Research Area | Cell Biology and Cellular Response antibody |
| Calculated MW | 29 kDa |
Images (2) Click the Picture to Zoom In
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ARG65301 anti-PSMA4 antibody WB image
Western Blot: K562 cell lysate (35µg protein in RIPA buffer) stained with ARG65301 anti-PSMA4 antibody (1µg/ml).
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ARG65301 anti-PSMA4 antibody WB image
Western Blot: NIH3T3 (A), Mouse Spleen (B), Rat Spleen (C)) lysates (35µg protein in RIPA buffer) stained with ARG65301 anti-PSMA4 antibody (0.3µg/ml)
