Overview

Product Description	Rabbit Polyclonal antibody recognizes PSMA3
Tested Reactivity	Hu, Ms, Rat
Tested Application	IHC-P, IP, WB
Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Target Name	PSMA3
Antigen Species	Human
Immunogen	Recombinant protein of Human PSMA3
Conjugation	Un-conjugated
Alternate Names	Proteasome subunit alpha type-3; Proteasome component C8; PSC3; HC8; Multicatalytic endopeptidase complex subunit C8; Macropain subunit C8; EC 3.4.25.1

Application Instructions

Application Suggestion	Tested Application Dilution IHC-P 1:50 - 1:200 IP Assay-dependent WB 1:500 - 1:2000
Application Note	* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Positive Control	PC3

Properties

Form	Liquid
Purification	Affinity purification with immunogen.
Buffer	PBS (pH 7.3), 0.02% Sodium azide and 50% Glycerol.
Preservative	0.02% Sodium azide
Stabilizer	50% Glycerol
Storage Instruction	For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note	For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links	GeneID: 19167 Mouse PSMA3 GeneID: 29670 Rat PSMA3 GeneID: 5684 Human PSMA3 Swiss-port # O70435 Mouse Proteasome subunit alpha type-3 Swiss-port # P18422 Rat Proteasome subunit alpha type-3 Swiss-port # P25788 Human Proteasome subunit alpha type-3 More
Gene Symbol	PSMA3
Gene Full Name	proteasome (prosome, macropain) subunit, alpha type, 3
Background	The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a member of the peptidase T1A family, that is a 20S core alpha subunit. Two alternative transcripts encoding different isoforms have been identified. [provided by RefSeq, Jul 2008]
Function	The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity. Binds to the C-terminus of CDKN1A and thereby mediates its degradation. Negatively regulates the membrane trafficking of the cell-surface thromboxane A2 receptor (TBXA2R) isoform 2. [UniProt]
Calculated MW	28 kDa

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