Overview

Product Description	Rabbit Polyclonal antibody recognizes PSMA1
Tested Reactivity	Hu, Ms, Rat
Tested Application	ICC/IF, WB
Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Target Name	PSMA1
Antigen Species	Human
Immunogen	Recombinant protein of Human PSMA1
Conjugation	Un-conjugated
Alternate Names	HC2; Proteasome subunit alpha type-1; Multicatalytic endopeptidase complex subunit C2; PROS30; Proteasome component C2; 30 kDa prosomal protein; PROS-30; EC 3.4.25.1; HEL-S-275; Proteasome nu chain; NU; Macropain subunit C2

Application Instructions

Application Suggestion	Tested Application Dilution ICC/IF 1:50 - 1:200 WB 1:500 - 1:2000
Application Note	* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Positive Control	U251

Properties

Form	Liquid
Purification	Affinity purification with immunogen.
Buffer	PBS (pH 7.3), 0.02% Sodium azide and 50% Glycerol.
Preservative	0.02% Sodium azide
Stabilizer	50% Glycerol
Storage Instruction	For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note	For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links	GeneID: 26440 Mouse PSMA1 GeneID: 29668 Rat PSMA1 GeneID: 5682 Human PSMA1 Swiss-port # P18420 Rat Proteasome subunit alpha type-1 Swiss-port # P25786 Human Proteasome subunit alpha type-1 Swiss-port # Q9R1P4 Mouse Proteasome subunit alpha type-1 More
Gene Symbol	PSMA1
Gene Full Name	proteasome (prosome, macropain) subunit, alpha type, 1
Background	The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a member of the peptidase T1A family, that is a 20S core alpha subunit. Alternative splicing results in multiple transcript variants encoding distinct isoforms.[provided by RefSeq, Jan 2009]
Function	The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity. Mediates the lipopolysaccharide-induced signal transduction in the macrophage proteasome (By similarity). Might be involved in the anti-inflammatory response of macrophages during the interaction with C.albicans heat-inactivated cells (By similarity). [UniProt]
Calculated MW	30 kDa

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