ARG43975

anti-PRPF31 / RP11 antibody

anti-PRPF31 / RP11 antibody for ELISA,Flow cytometry,ICC/IF,Western blot and Human,Mouse,Rat

Overview

Product Description Rabbit Polyclonal antibody recognizes PRPF31 / RP11
Tested Reactivity Hu, Ms, Rat
Tested Application ELISA, FACS, ICC/IF, WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name PRPF31 / RP11
Antigen Species Human
Immunogen Human PRPF31 / RP11 recombinant protein
Expression System E.coli
Conjugation Un-conjugated
Protein Full Name U4/U6 small nuclear ribonucleoprotein Prp31
Alternate Names PRPF31; Pre-MRNA Processing Factor 31; HPrp31; PRP31; U4/U6 Small Nuclear Ribonucleoprotein Prp31; NY-BR-99; SNRNP61; Serologically Defined Breast Cancer Antigen NY-BR-99; U4/U6 SnRNP 61 KDa Protein; Protein 61K; RP11; PRP31 Pre-MRNA Processing Factor 31 Homolog; Pre-MRNA-Processing Factor 31

Application Instructions

Application Suggestion
Tested Application Dilution
ELISA0.1-0.5 µg/ml
FACS1-3 µg/1x10^6
ICC/IF5 µg/ml
WB0.25-0.5 µg/ml
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Buffer 0.9% NaCl, 0.2% Na2HPO4 and 4% Trehalose.
Stabilizer 4% Trehalose
Concentration 0.5 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.

Bioinformation

Database Links

GeneID: 26121 Human PRPF31

GeneID: 68988 Mouse PRPF31

Swiss-port # Q8CCF0 Mouse U4/U6 small nuclear ribonucleoprotein Prp31

Swiss-port # Q8WWY3 Human U4/U6 small nuclear ribonucleoprotein Prp31

Gene Symbol PRPF31
Gene Full Name Pre-MRNA Processing Factor 31
Background This gene encodes a component of the spliceosome complex and is one of several retinitis pigmentosa-causing genes. When the gene product is added to the spliceosome complex, activation occurs.
Function Required for the assembly of the U4/U5/U6 tri-snRNP complex, one of the building blocks of the spliceosome.
Cellular Localization Nucleus, Spliceosome
Calculated MW 55 kDa
PTM Acetylation, Isopeptide bond, Phosphoprotein, Ubl conjugation

Images (5) Click the Picture to Zoom In

  • ARG43975 anti-PRPF31 / RP11 antibody ICC/IF image

    Immunofluorescence: A549 cells stained with ARG43975 anti-PRPF31 / RP11 antibody at 5 μg/ml dilution.

  • ARG43975 anti-PRPF31 / RP11 antibody WB image

    Western blot: Hela, 293T and A549 stained with ARG43975 anti-PRPF31 / RP11 antibody at 0.5 μg/mL dilution.

  • ARG43975 anti-PRPF31 / RP11 antibody FACS image

    Flow Cytometry: U937 cells stained with ARG43975 anti-PRPF31 / RP11 antibody (blue) at 1 μg/1x10^6 cells dilution.

  • ARG43975 anti-PRPF31 / RP11 antibody WB image

    Western blot: C6 stained with ARG43975 anti-PRPF31 / RP11 antibody at 0.5 μg/mL dilution.

  • ARG43975 anti-PRPF31 / RP11 antibody WB image

    Western blot: NIH/3T3 stained with ARG43975 anti-PRPF31 / RP11 antibody at 0.5 μg/mL dilution.