ARG43973

anti-PRPF18 antibody

anti-PRPF18 antibody for ELISA,Flow cytometry,ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat

Overview

Product Description Rabbit Polyclonal antibody recognizes PRPF18
Tested Reactivity Hu, Ms, Rat
Tested Application ELISA, FACS, ICC/IF, IHC-P, WB
Host Rabbit
Clonality Polyclonal
Target Name PRPF18
Antigen Species Human
Immunogen Human PRPF18 recombinant protein
Expression System E.coli
Conjugation Un-conjugated
Protein Full Name Pre-mRNA-splicing factor 18
Alternate Names PRPF18; Pre-MRNA Processing Factor 18; HPrp18; Pre-MRNA-Splicing Factor 18; PRP18 Homolog; HPRP18; PRP18 Pre-MRNA Processing Factor 18 Homolog; PRP18

Application Instructions

Application Suggestion
Tested Application Dilution
ELISA0.1-0.5 µg/ml
FACS1-3 µg/1x10^6
ICC/IF5 µg/ml
IHC-P2-5 µg/ml
WB0.25-0.5 µg/ml
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Buffer 0.9% NaCl, 0.2% Na2HPO4 and 4% Trehalose.
Stabilizer 4% Trehalose
Concentration 0.5 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.

Bioinformation

Database Links

GeneID: 171552 Rat PRPF18

GeneID: 67229 Mouse PRPF18

GeneID: 8559 Human PRPF18

Gene Symbol PRPF18
Gene Full Name Pre-MRNA Processing Factor 18
Background Pre-mRNA splicing occurs in 2 sequential transesterification steps. The protein encoded by this gene is found to be essential for the catalytic step II in pre-mRNA splicing process. It is found in the spliceosome, and contains seven WD repeats, which function in protein-protein interactions. This protein has a sequence similarity to the yeast splicing factor Prp18.
Function Participates in the second step of pre-mRNA splicing.
Cellular Localization Nucleus; Spliceosome
Calculated MW 40 kDa
PTM Acetylation

Images (8) Click the Picture to Zoom In

  • ARG43973 anti-PRPF18 antibody IHC-P image

    Immunohistochemistry: Human urothelial carcinoma stained with ARG43973 anti-PRPF18 antibody at 2 μg/ml dilution.

  • ARG43973 anti-PRPF18 antibody ICC/IF image

    Immunofluorescence: A549 cells stained with ARG43973 anti-PRPF18 antibody at 5 μg/ml dilution.

  • ARG43973 anti-PRPF18 antibody WB image

    Western blot: SH-SY5Y stained with ARG43973 anti-PRPF18 antibody at 0.5 μg/mL dilution.

  • ARG43973 anti-PRPF18 antibody FACS image

    Flow Cytometry: JK cells stained with ARG43973 anti-PRPF18 antibody (blue) at 1 μg/1x10^6 cells dilution.

  • ARG43973 anti-PRPF18 antibody IHC-P image

    Immunohistochemistry: Rat testis stained with ARG43973 anti-PRPF18 antibody at 2 μg/ml dilution.

  • ARG43973 anti-PRPF18 antibody WB image

    Western blot: PC-12 stained with ARG43973 anti-PRPF18 antibody at 0.5 μg/mL dilution.

  • ARG43973 anti-PRPF18 antibody IHC-P image

    Immunohistochemistry: Mouse testis stained with ARG43973 anti-PRPF18 antibody at 2 μg/ml dilution.

  • ARG43973 anti-PRPF18 antibody WB image

    Western blot: Mouse brain stained with ARG43973 anti-PRPF18 antibody at 0.5 μg/mL dilution.