ARG44069

anti-PRMT7 antibody

anti-PRMT7 antibody for Flow cytometry,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat

Gene Regulation antibody

Overview

Product Description Rabbit Polyclonal recognizes PRMT7
Tested Reactivity Hu, Ms, Rat
Tested Application FACS, IHC-P, WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name PRMT7
Antigen Species Human
Immunogen Human PRMT7 recombinant protein (Position: K121-D526).
Conjugation Un-conjugated
Alternate Names PRMT7; Protein Arginine Methyltransferase 7; KIAA1933; [Myelin Basic Protein]-Arginine N-Methyltransferase PRMT7; Histone-Arginine N-Methyltransferase PRMT7; Protein Arginine N-Methyltransferase 7; FLJ10640; EC 2.1.1.321; EC 2.1.1; SBIDDS

Application Instructions

Application Suggestion
Tested Application Dilution
FACS1 - 3 µg/1x10^6 cells
IHC-P2 - 5 µg/ml
WB0.25 - 0.5 µg/ml
Application Note The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Affinity purification with immunogen.
Buffer 0.9% NaCl, 0.2% Na2HPO4, 0.05% Sodium azide and 4% Trehalose.
Preservative 0.05% Sodium azide
Stabilizer 4% Trehalose
Concentration 0.5 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 214572 Mouse PRMT7

GeneID: 361402 Rat PRMT7

GeneID: 54496 Human PRMT7

Gene Symbol PRMT7
Gene Full Name Protein Arginine Methyltransferase 7
Background Arginine methylation is an apparently irreversible protein modification catalyzed by arginine methyltransferases, such as PMT7, using S-adenosylmethionine (AdoMet) as the methyl donor. Arginine methylation is implicated in signal transduction, RNA transport, and RNA splicing (Miranda et al., 2004 [PubMed 15044439]).[supplied by OMIM, Mar 2008]
Function Arginine methyltransferase that can both catalyze the formation of omega-N monomethylarginine (MMA) and symmetrical dimethylarginine (sDMA), with a preference for the formation of MMA. Specifically mediates the symmetrical dimethylation of arginine residues in the small nuclear ribonucleoproteins Sm D1 (SNRPD1) and Sm D3 (SNRPD3); such methylation being required for the assembly and biogenesis of snRNP core particles. Specifically mediates the symmetric dimethylation of histone H4 'Arg-3' to form H4R3me2s. Plays a role in gene imprinting by being recruited by CTCFL at the H19 imprinted control region (ICR) and methylating histone H4 to form H4R3me2s, possibly leading to recruit DNA methyltransferases at these sites. May also play a role in embryonic stem cell (ESC) pluripotency. Also able to mediate the arginine methylation of histone H2A and myelin basic protein (MBP) in vitro; the relevance of such results is however unclear in vivo.
Cellular Localization Cytoplasm, Nucleus
Research Area Gene Regulation antibody
Calculated MW 78 kDa
PTM Methylation

Images (5) Click the Picture to Zoom In

  • ARG44069 anti-PRMT7 antibody IHC-P image

    Immunohistochemistry: Human ovarian cancer stained with ARG44069 anti-PRMT7 antibody at 2 μg/mL dilution.

  • ARG44069 anti-PRMT7 antibody WB image

    Western blot: Hela stained with ARG44069 anti-PRMT7 antibody at 0.5 μg/mL dilution.

  • ARG44069 anti-PRMT7 antibody FACS image

    Flow Cytometry: HepG2 stained with ARG44069 anti-PRMT7 antibody at 1 μg/1x10^6 cells dilution.

  • ARG44069 anti-PRMT7 antibody IHC-P image

    Immunohistochemistry: Rat testis stained with ARG44069 anti-PRMT7 antibody at 2 μg/mL dilution.

  • ARG44069 anti-PRMT7 antibody IHC-P image

    Immunohistochemistry: Mouse testis stained with ARG44069 anti-PRMT7 antibody at 2 μg/mL dilution.