ARG43933
anti-PRMT1 antibody
anti-PRMT1 antibody for ELISA,Flow cytometry,ICC/IF,Western blot and Human,Mouse,Rat
Overview
| Product Description | Rabbit Polyclonal antibody recognizes PRMT1 |
|---|---|
| Tested Reactivity | Hu, Ms, Rat |
| Tested Application | ELISA, FACS, ICC/IF, WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | PRMT1 |
| Antigen Species | Human |
| Immunogen | Human PRMT1 recombinant protein |
| Expression System | E.coli |
| Conjugation | Un-conjugated |
| Protein Full Name | Protein arginine N-methyltransferase 1 |
| Alternate Names | PRMT1; Protein Arginine Methyltransferase 1; HRMT1L2; HCP1; ANM1; HMT1 (HnRNP Methyltransferase, S. Cerevisiae)-Like 2; Histone-Arginine N-Methyltransferase PRMT1; Protein Arginine N-Methyltransferase 1; Interferon Receptor 1-Bound Protein 4; Highly Conserved Protein 1; IR1B4; Heterogeneous Nuclear Ribonucleoprotein Methyltransferase 1-Like 2; HMT1 HnRNP Methyltransferase-Like 2 (S. Cerevisiae); EC 2.1.1.319; EC 2.1.1.77; EC 2.1.1; HMT2 |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purified with Immunogen. |
| Buffer | 0.9% NaCl, 0.2% Na2HPO4 and 4% Trehalose. |
| Stabilizer | 4% Trehalose |
| Concentration | 0.5 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | PRMT1 |
| Gene Full Name | Protein Arginine Methyltransferase 1 |
| Background | This gene encodes a member of the protein arginine N-methyltransferase (PRMT) family. Post-translational modification of target proteins by PRMTs plays an important regulatory role in many biological processes, whereby PRMTs methylate arginine residues by transferring methyl groups from S-adenosyl-L-methionine to terminal guanidino nitrogen atoms. The encoded protein is a type I PRMT and is responsible for the majority of cellular arginine methylation activity. Increased expression of this gene may play a role in many types of cancer. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene, and a pseudogene of this gene is located on the long arm of chromosome 5. |
| Function | Arginine methyltransferase that methylates (mono and asymmetric dimethylation) the guanidino nitrogens of arginyl residues present in proteins such as ESR1, histone H2, H3 and H4, FMR1, ILF3, HNRNPA1, HNRNPD, NFATC2IP, SUPT5H, TAF15, EWS, HABP4, SERBP1, RBM15, FOXO1, CHTOP and MAP3K5/ASK1. |
| Cellular Localization | Cytoplasm; Nucleus |
| Calculated MW | 42 kDa |
| PTM | Acetylation; Isopeptide bond; Phosphoprotein; Ubl conjugation |
Images (5) Click the Picture to Zoom In
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ARG43933 anti-PRMT1 antibody ICC/IF image
Immunofluorescence: A549 cells stained with ARG43933 anti-PRMT1 antibody at 5 μg/ml dilution.
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ARG43933 anti-PRMT1 antibody WB image
Western blot: HepG2, A549 and Hela stained with ARG43933 anti-PRMT1 antibody at 0.5 μg/mL dilution.
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ARG43933 anti-PRMT1 antibody FACS image
Flow Cytometry: U937 cells stained with ARG43933 anti-PRMT1 antibody (blue) at 1 μg/1x10^6 cells dilution.
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ARG43933 anti-PRMT1 antibody WB image
Western blot: RH35 stained with ARG43933 anti-PRMT1 antibody at 0.5 μg/mL dilution.
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ARG43933 anti-PRMT1 antibody WB image
Western blot: Neuro-2a stained with ARG43933 anti-PRMT1 antibody at 0.5 μg/mL dilution.
