ARG43953
anti-PREX2 antibody
anti-PREX2 antibody for ELISA,Western blot and Human
Overview
| Product Description | Rabbit Polyclonal antibody recognizes PREX2 |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | ELISA, WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Target Name | PREX2 |
| Antigen Species | Human |
| Immunogen | Human PREX2 recombinant protein |
| Expression System | E.coli |
| Conjugation | Un-conjugated |
| Protein Full Name | Phosphatidylinositol 3,4,5-trisphosphate-dependent Rac exchanger 2 protein |
| Alternate Names | PREX2; Phosphatidylinositol-3,4,5-Trisphosphate Dependent Rac Exchange Factor 2; PPP1R129; P-REX2; DEPDC2; DEP.2; Phosphatidylinositol 3,4,5-Trisphosphate-Dependent Rac Exchanger 2 Protein; Protein Phosphatase 1, Regulatory Subunit 129; PtdIns(3,4,5)-Dependent Rac Exchanger 2; DEP Domain-Containing Protein 2; FLJ12987; Phosphatidylinositol-3,4,5-Trisphosphate-Dependent Rac Exchange Factor 2; DEP Domain Containing 2; P-Rex2 |
Application Instructions
| Application Suggestion |
|
||||||
|---|---|---|---|---|---|---|---|
| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Buffer | 0.9% NaCl, 0.2% Na2HPO4 and 4% Trehalose. |
| Stabilizer | 4% Trehalose |
| Concentration | 0.5 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
Bioinformation
| Database Links |
Swiss-port # Q70Z35 Human Phosphatidylinositol 3,4,5-trisphosphate-dependent Rac exchanger 2 protein |
|---|---|
| Gene Symbol | PREX2 |
| Gene Full Name | Phosphatidylinositol-3,4,5-Trisphosphate Dependent Rac Exchange Factor 2 |
| Background | The protein encoded by this gene belongs to the phosphatidylinositol 3,4,5-trisphosphate (PIP3)-dependent Rac exchanger (PREX) family, which are Dbl-type guanine-nucleotide exchange factors for Rac family small G proteins. Structural domains of this protein include the catalytic diffuse B-cell lymphoma homology and pleckstrin homology (DHPH) domain, two disheveled, EGL-10, and pleckstrin homology (DEP) domains, two PDZ domains, and a C-terminal inositol polyphosphate-4 phosphatase (IP4P) domain that is found in one of the isoforms. This protein facilitates the exchange of GDP for GTP on Rac1, allowing the GTP-bound Rac1 to activate downstream effectors. Studies also show that the pleckstrin homology domain of this protein interacts with the phosphatase and tensin homolog (PTEN) gene product to inhibit PTEN phosphatase activity, thus activating the phosphoinositide-3 kinase (PI3K) signaling pathway. Conversely, the PTEN gene product has also been shown to inhibit the GEF activity of this protein. This gene plays a role in insulin-signaling pathways, and either mutations or overexpression of this gene have been observed in some cancers. |
| Function | Functions as a RAC1 guanine nucleotide exchange factor (GEF), activating Rac proteins by exchanging bound GDP for free GTP. Its activity is synergistically activated by phosphatidylinositol 3,4,5-trisphosphate and the beta gamma subunits of heterotrimeric G protein. Mediates the activation of RAC1 in a PI3K-dependent manner. May be an important mediator of Rac signaling, acting directly downstream of both G protein-coupled receptors and phosphoinositide 3-kinase. |
| Cellular Localization | Cytosol, Plasma membrane |
| Calculated MW | 183 kDa |
Images (1) Click the Picture to Zoom In
-
ARG43953 anti-PREX2 antibody WB image
Western blot: Mouse skeletal muscle stained with ARG43953 anti-PREX2 antibody at 0.5 μg/mL dilution.
