ARG56962

anti-PPM1G / PP2C gamma antibody [k1G6]

anti-PPM1G / PP2C gamma antibody [k1G6] for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human

Overview

Product Description Mouse Monoclonal antibody [k1G6] recognizes PPM1G / PP2C gamma
Tested Reactivity Hu
Tested Application IHC-P, WB
Host Mouse
Clonality Monoclonal
Clone k1G6
Isotype IgG1, kappa
Target Name PPM1G / PP2C gamma
Antigen Species Human
Immunogen Recombinant fragment around aa. 317-546 of Human PPM1G / PP2C gamma.
Conjugation Un-conjugated
Alternate Names Protein phosphatase magnesium-dependent 1 gamma; PP2CGAMMA; PPP2CG; EC 3.1.3.16; Protein phosphatase 1C; Protein phosphatase 1G; Protein phosphatase 2C isoform gamma; PP2CG; PP2C-gamma

Application Instructions

Application Suggestion
Tested Application Dilution
IHC-P1:50
WB1:1000 - 1:2000
Application Note IHC-P: Antigen Retrieval: Boil tissue section in 0.1M Sodium citrate buffer (pH 6.0) for 20 min.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Purification with Protein G.
Buffer PBS (pH 7.4), 0.02% Sodium azide and 10% Glycerol.
Preservative 0.02% Sodium azide
Stabilizer 10% Glycerol
Concentration 1 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 5496 Human PPM1G

Swiss-port # O15355 Human Protein phosphatase 1G

Gene Symbol PPM1G
Gene Full Name protein phosphatase, Mg2+/Mn2+ dependent, 1G
Background The protein encoded by this gene is a member of the PP2C family of Ser/Thr protein phosphatases. PP2C family members are known to be negative regulators of cell stress response pathways. This phosphatase is found to be responsible for the dephosphorylation of Pre-mRNA splicing factors, which is important for the formation of functional spliceosome. Studies of a similar gene in mice suggested a role of this phosphatase in regulating cell cycle progression. [provided by RefSeq, Apr 2010]
Calculated MW 59 kDa

Images (2) Click the Picture to Zoom In

  • ARG56962 anti-PPM1G / PP2C gamma antibody [k1G6] IHC-P image

    Immunohistochemistry: Paraffin embedded sections of Human breast cancer tissue stained with ARG56962 anti-PPM1G / PP2C gamma antibody [k1G6] at 1:50 for 2 hours at RT. Antigen Retrieval: Boil tissue section in 0.1M Sodium citrate buffer (pH 6.0) for 20 min.

  • ARG56962 anti-PPM1G / PP2C gamma antibody [k1G6] WB image

    Western blot: 20 µg of 293T cell lysate stained with ARG56962 anti-PPM1G / PP2C gamma antibody [k1G6] at 1:1000.