ARG57082
anti-PPCS antibody [10D11]
anti-PPCS antibody [10D11] for Western blot and Human
Overview
| Product Description | Mouse Monoclonal antibody [10D11] recognizes PPCS |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | WB |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | 10D11 |
| Isotype | IgG1, kappa |
| Target Name | PPCS |
| Antigen Species | Human |
| Immunogen | Recombinant fragment around aa. 1-311 of Human PPCS. |
| Conjugation | Un-conjugated |
| Alternate Names | Phosphopantothenoylcysteine synthetase; PPC synthetase; EC 6.3.2.5; Phosphopantothenate--cysteine ligase |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purification with Protein A. |
| Buffer | PBS (pH 7.4), 0.02% Sodium azide and 10% Glycerol. |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 10% Glycerol |
| Concentration | 1 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # Q9HAB8 Human Phosphopantothenate--cysteine ligase |
|---|---|
| Gene Symbol | PPCS |
| Gene Full Name | phosphopantothenoylcysteine synthetase |
| Background | Biosynthesis of coenzyme A (CoA) from pantothenic acid (vitamin B5) is an essential universal pathway in prokaryotes and eukaryotes. PPCS (EC 6.3.2.5), one of the last enzymes in this pathway, converts phosphopantothenate to phosphopantothenoylcysteine (Daugherty et al., 2002 [PubMed 11923312]).[supplied by OMIM, Mar 2008] |
| Function | Catalyzes the first step in the biosynthesis of coenzyme A from vitamin B5, where cysteine is conjugated to 4'-phosphopantothenate to form 4-phosphopantothenoylcysteine. [UniProt] |
| Calculated MW | 34 kDa |
Images (1) Click the Picture to Zoom In
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ARG57082 anti-PPCS antibody [10D11] WB image
Western blot: 40 µg of 1) Jurkat cell lysate, 2) HepG2 cell lysate, 3) HeLa cell lysate, 4) A549 cell lysate, 5) MCF7 cell lysate, 6) SK-OV-3 cell lysate stained with ARG57082 anti-PPCS antibody [10D11] at 1:1000.
