ARG63625
anti-PPAR delta antibody
anti-PPAR delta antibody for Western blot and Human
Cell Biology and Cellular Response antibody; Gene Regulation antibody; Metabolism antibody
Overview
| Product Description | Goat Polyclonal antibody recognizes PPAR delta |
|---|---|
| Tested Reactivity | Hu |
| Predict Reactivity | Ms, Rat, Cow, Pig |
| Tested Application | WB |
| Specificity | This antibody is expected to recognise isoform 1 (NP_006229) of PPAR delta, but not isoform 2, a shorter protein (NP_803184) with a different C-terminus. |
| Host | Goat |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | PPAR delta |
| Antigen Species | Human |
| Immunogen | CHPLLQEIYKDMY |
| Conjugation | Un-conjugated |
| Alternate Names | PPAR-delta; NR1C2; PPAR-beta; PPARB; NUCII; NUCI; Nuclear receptor subfamily 1 group C member 2; Nuclear hormone receptor 1; NUC1; Peroxisome proliferator-activated receptor delta; FAAR; Peroxisome proliferator-activated receptor beta |
Application Instructions
| Application Suggestion |
|
||||
|---|---|---|---|---|---|
| Application Note | WB: Recommend incubate at RT for 1h. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Buffer | Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 0.5% BSA |
| Concentration | 0.5 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # Q03181 Human Peroxisome proliferator-activated receptor delta |
|---|---|
| Background | This gene encodes a member of the peroxisome proliferator-activated receptor (PPAR) family. PPARs are nuclear hormone receptors that bind peroxisome proliferators and control the size and number of peroxisomes produced by cells. PPARs mediate a variety of biological processes, and may be involved in the development of several chronic diseases, including diabetes, obesity, atherosclerosis, and cancer. This protein is a potent inhibitor of ligand-induced transcription activity of PPAR alpha and PPAR gamma. It may function as an integrator of transcription repression and nuclear receptor signaling. The expression of this gene is found to be elevated in colorectal cancer cells. The elevated expression can be repressed by adenomatosis polyposis coli (APC), a tumor suppressor protein related to APC/beta-catenin signaling pathway. Knockout studies in mice suggested the role of this protein in myelination of the corpus callosum, lipid metabolism, and epidermal cell proliferation. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Jan 2010] |
| Research Area | Cell Biology and Cellular Response antibody; Gene Regulation antibody; Metabolism antibody |
| Calculated MW | 50 kDa |
Images (1) Click the Picture to Zoom In
-
ARG63625 anti-PPAR delta antibody WB image
Western blot: 35 µg of MOLT-4 cell lysate (in RIPA buffer) stained with ARG63625 anti-PPAR delta antibody at 0.3 µg/ml dilution and incubated at RT for 1 hour.
