ARG65249

anti-POU6F2 antibody

anti-POU6F2 antibody for Western blot and Mouse

Cancer antibody; Gene Regulation antibody

Overview

Product Description Goat Polyclonal antibody recognizes POU6F2
Tested Reactivity Ms
Predict Reactivity Hu, Rat, Cow
Tested Application WB
Specificity This antibody is expected to recognize both reported isoforms (NP_009183.3; NP_001159490.1).
Host Goat
Clonality Polyclonal
Isotype IgG
Target Name POU6F2
Antigen Species Human
Immunogen RSEMNAELRGEDK-C
Conjugation Un-conjugated
Alternate Names WTSL; POU domain, class 6, transcription factor 2; Retina-derived POU domain factor 1; WT5; RPF-1

Application Instructions

Application Suggestion
Tested Application Dilution
WB0.3 - 1 µg/ml
Application Note WB: Recommend incubate at RT for 1h.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Buffer Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA
Preservative 0.02% Sodium azide
Stabilizer 0.5% BSA
Concentration 0.5 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 218030 Mouse POU6F2

Swiss-port # Q8BJI4 Mouse POU domain, class 6, transcription factor 2

Background This gene encodes a member of the POU protein family characterized by the presence of a bipartite DNA binding domain, consisting of a POU-specific domain and a homeodomain, separated by a variable polylinker. The DNA binding domain may bind to DNA as monomers or as homo- and/or heterodimers, in a sequence-specific manner. The POU family members are transcriptional regulators, many of which are known to control cell type-specific differentiation pathways. This gene is a tumor suppressor involved in Wilms tumor (WT) predisposition. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene.[provided by RefSeq, Oct 2009]
Research Area Cancer antibody; Gene Regulation antibody
Calculated MW 73 kDa

Images (1) Click the Picture to Zoom In

  • ARG65249 anti-POU6F2 antibody WB image

    Western Blot: Mouse Eye lysate (35 µg protein in RIPA buffer) stained with ARG65249 anti-POU6F2 antibody at 0.3 µg/ml dilution.