ARG65233

anti-POU3F3 / BRN1 / OCT8 antibody

anti-POU3F3 / BRN1 / OCT8 antibody for Western blot and Mouse

Gene Regulation antibody; Neuroscience antibody

Overview

Product Description Goat Polyclonal antibody recognizes POU3F3 / BRN1 / OCT8
Tested Reactivity Ms
Predict Reactivity Hu, Rat
Tested Application WB
Host Goat
Clonality Polyclonal
Isotype IgG
Target Name POU3F3 / BRN1 / OCT8
Antigen Species Human
Immunogen C-HMLSHAHQWVTAL
Conjugation Un-conjugated
Alternate Names Oct-8; BRN1; Octamer-binding transcription factor 8; brain-1; Brn-1; OTF-8; Octamer-binding protein 8; Brain-specific homeobox/POU domain protein 1; oct-8; POU domain, class 3, transcription factor 3; OTF8; Brain-1

Application Instructions

Application Suggestion
Tested Application Dilution
WB1 - 3 µg/ml
Application Note WB: Recommend incubate at RT for 1h.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Buffer Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA
Preservative 0.02% Sodium azide
Stabilizer 0.5% BSA
Concentration 0.5 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 18993 Mouse POU3F3

Swiss-port # P31361 Mouse POU domain, class 3, transcription factor 3

Background POU3F3 is a member of the class III POU family of transcription factors (see POU3F1; MIM 602479) that are expressed in the central nervous system. The POU domain in these proteins is required for high affinity binding to octamer DNA sequences (summarized by Sumiyama et al., 1996 [PubMed 8703082]).[supplied by OMIM, Jul 2010]
Research Area Gene Regulation antibody; Neuroscience antibody
Calculated MW 50 kDa

Images (1) Click the Picture to Zoom In

  • ARG65233 anti-POU3F3 / BRN1 / OCT8 antibody WB image

    Western Blot: Mouse Spinal Cord lysate (35 µg protein in RIPA buffer) stained with ARG65233 anti-POU3F3 / BRN1 / OCT8 antibody at 1 µg/ml dilution.