ARG41571
anti-PON1 / paraoxonase 1 antibody
anti-PON1 / paraoxonase 1 antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Mouse,Rat
Overview
| Product Description | Rabbit Polyclonal antibody recognizes PON1 / paraoxonase 1 |
|---|---|
| Tested Reactivity | Ms, Rat |
| Tested Application | IHC-P, WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | PON1 / paraoxonase 1 |
| Antigen Species | Rat |
| Immunogen | Recombinant protein corresponding to A30-D274 of Rat PON1 / paraoxonase 1. |
| Conjugation | Un-conjugated |
| Alternate Names | K-45; Aromatic esterase 1; MVCD5; EC 3.1.1.81; A-esterase 1; PON; Serum paraoxonase/arylesterase 1; ESA; Serum aryldialkylphosphatase 1; EC 3.1.8.1; EC 3.1.1.2; PON 1 |
Application Instructions
| Application Suggestion |
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| Application Note | IHC-P: Antigen Retrieval: Heat mediation was performed in Citrate buffer (pH 6.0, epitope retrieval solution) for 20 min. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
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| Observed Size | ~ 43 kDa |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purification with immunogen. |
| Buffer | 0.2% Na2HPO4, 0.9% NaCl, 0.05% Sodium azide and 4% Trehalose. |
| Preservative | 0.05% Sodium azide |
| Stabilizer | 4% Trehalose |
| Concentration | 0.5 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | PON1 |
| Gene Full Name | paraoxonase 1 |
| Background | The enzyme encoded by this gene is an arylesterase that mainly hydrolyzes paroxon to produce p-nitrophenol. Paroxon is an organophosphorus anticholinesterase compound that is produced in vivo by oxidation of the insecticide parathion. Polymorphisms in this gene are a risk factor in coronary artery disease. The gene is found in a cluster of three related paraoxonase genes at 7q21.3. [provided by RefSeq, Oct 2008] |
| Function | Hydrolyzes the toxic metabolites of a variety of organophosphorus insecticides. Capable of hydrolyzing a broad spectrum of organophosphate substrates and lactones, and a number of aromatic carboxylic acid esters. Mediates an enzymatic protection of low density lipoproteins against oxidative modification and the consequent series of events leading to atheroma formation. [UniProt] |
| Cellular Localization | Secreted, extracellular space. [UniProt] |
| Calculated MW | 40 kDa |
| PTM | Glycosylated. The signal sequence is not cleaved. Present in two forms, form B contains a disulfide bond, form A does not. [UniProt] |
Images (5) Click the Picture to Zoom In
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ARG41571 anti-PON1 / paraoxonase 1 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Rat kidney tissue. Antigen Retrieval: Heat mediation was performed in Citrate buffer (pH 6.0, epitope retrieval solution) for 20 min. The tissue section was blocked with 10% goat serum. The tissue section was then stained with ARG41571 anti-PON1 / paraoxonase 1 antibody at 2 µg/ml dilution, overnight at 4°C.
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ARG41571 anti-PON1 / paraoxonase 1 antibody WB image
Western blot: 50 µg of samples under reducing conditions. Rat liver and Mouse liver lysates stained with ARG41571 anti-PON1 / paraoxonase 1 antibody at 0.5 µg/ml dilution, overnight at 4°C.
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ARG41571 anti-PON1 / paraoxonase 1 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Mouse kidney tissue. Antigen Retrieval: Heat mediation was performed in Citrate buffer (pH 6.0, epitope retrieval solution) for 20 min. The tissue section was blocked with 10% goat serum. The tissue section was then stained with ARG41571 anti-PON1 / paraoxonase 1 antibody at 2 µg/ml dilution, overnight at 4°C.
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ARG41571 anti-PON1 / paraoxonase 1 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Rat liver tissue. Antigen Retrieval: Heat mediation was performed in Citrate buffer (pH 6.0, epitope retrieval solution) for 20 min. The tissue section was blocked with 10% goat serum. The tissue section was then stained with ARG41571 anti-PON1 / paraoxonase 1 antibody at 2 µg/ml dilution, overnight at 4°C.
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ARG41571 anti-PON1 / paraoxonase 1 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Rat small intestine tissue. Antigen Retrieval: Heat mediation was performed in Citrate buffer (pH 6.0, epitope retrieval solution) for 20 min. The tissue section was blocked with 10% goat serum. The tissue section was then stained with ARG41571 anti-PON1 / paraoxonase 1 antibody at 2 µg/ml dilution, overnight at 4°C.
