ARG59947
anti-POLR2F antibody
anti-POLR2F antibody for Western blot and Human,Rat
Overview
| Product Description | Rabbit Polyclonal antibody recognizes POLR2F |
|---|---|
| Tested Reactivity | Hu, Rat |
| Tested Application | WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | POLR2F |
| Antigen Species | Human |
| Immunogen | Recombinant fusion protein corresponding to aa. 1-127 of Human POLR2F (NP_068809.1). |
| Conjugation | Un-conjugated |
| Alternate Names | POLRF; RPB6; RPB6 homolog; HRBP14.4; DNA-directed RNA polymerases I, II, and III subunit RPABC2; RPABC2; RPB14.4; DNA-directed RNA polymerase II subunit F; RPC15; RPABC14.4; RNA polymerases I, II, and III subunit ABC2; DNA-directed RNA polymerases I, II, and III 14.4 kDa polypeptide |
Application Instructions
| Application Suggestion |
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|---|---|---|---|---|---|
| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||
| Positive Control | Rat heart | ||||
| Observed Size | 14 kDa |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purified. |
| Buffer | PBS (pH 7.3), 0.02% Sodium azide and 50% Glycerol. |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 50% Glycerol |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # O88828 Rat DNA-directed RNA polymerases I, II, and III subunit RPABC2 Swiss-port # P61218 Human DNA-directed RNA polymerases I, II, and III subunit RPABC2 |
|---|---|
| Gene Symbol | POLR2F |
| Gene Full Name | polymerase (RNA) II (DNA directed) polypeptide F |
| Background | This gene encodes the sixth largest subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. In yeast, this polymerase subunit, in combination with at least two other subunits, forms a structure that stabilizes the transcribing polymerase on the DNA template. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2014] |
| Function | DNA-dependent RNA polymerases catalyze the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Common component of RNA polymerases I, II, and III which synthesize ribosomal RNA precursors, mRNA precursors and many functional non-coding RNAs, and small RNAs, such as 5S rRNA and tRNAs, respectively. Pol II is the central component of the basal RNA polymerase II transcription machinery. Pols are composed of mobile elements that move relative to each other. In Pol II, POLR2F/RPB6 is part of the clamp element and together with parts of RPB1 and RPB2 forms a pocket to which the RPB4-RPB7 subcomplex binds (By similarity). [UniProt] |
| Cellular Localization | Nucleus. [UniProt] |
| Calculated MW | 14 kDa |
Images (1) Click the Picture to Zoom In
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ARG59947 anti-POLR2F antibody WB image
Western blot: 25 µg of Rat heart lysate stained with ARG59947 anti-POLR2F antibody at 1:3000 dilution.
