ARG63382
anti-POLL / POLkappa antibody
anti-POLL / POLkappa antibody for Western blot and Human
Gene Regulation antibody
Overview
| Product Description | Goat Polyclonal antibody recognizes POLL / POLkappa |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | WB |
| Specificity | It has been brought to our attention that this product may crossreact with Pol beta (NP_002681), an evolutionarilly related polymerase with a predicted size of 39 kDa. The overall % of identity is not very high (about 32% in the core) but considering only the peptide that we used as immunogen the two share the sequence YREP**R** at the C-terminus. Further the protein structures appear to be very similar in this region. |
| Host | Goat |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | POLL / POLkappa |
| Antigen Species | Human |
| Immunogen | C-LGLPYREPAERDW |
| Conjugation | Un-conjugated |
| Alternate Names | DNA polymerase beta-2; BETAN; EC 4.2.99.-; EC 2.7.7.7; Pol beta2; Pol Lambda; POLKAPPA; DNA polymerase kappa; DNA polymerase lambda |
Application Instructions
| Application Suggestion |
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|---|---|---|---|---|---|
| Application Note | WB: Recommend incubate at RT for 1h. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Buffer | Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 0.5% BSA |
| Concentration | 0.5 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Background | This gene encodes a DNA polymerase. DNA polymerases catalyze DNA-template-directed extension of the 3'-end of a DNA strand. This particular polymerase, which is a member of the X family of DNA polymerases, likely plays a role in non-homologous end joining and other DNA repair processes. Alternatively spliced transcript variants have been described. [provided by RefSeq, Mar 2010] |
| Research Area | Gene Regulation antibody |
| Calculated MW | 63 kDa |
Images (1) Click the Picture to Zoom In
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ARG63382 anti-POLL / POLkappa antibody WB image
Western blot: 35 µg of A549 (A) and HepG2 (B) cell lysates (in RIPA buffer) stained with ARG63382 anti-POLL / POLkappa antibody at 0.5 µg/ml (A) and 1 µg/ml (B) dilutions and incubated at RT for 1 hour.
