ARG42143
anti-POFUT2 antibody
anti-POFUT2 antibody for IHC-Formalin-fixed paraffin-embedded sections and Human
Overview
| Product Description | Rabbit Polyclonal antibody recognizes POFUT2 |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | IHC-P |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | POFUT2 |
| Antigen Species | Human |
| Immunogen | Synthetic peptide around the C-terminal region of Human POFUT2. (within the following region: RFEPT WEELE LYKDG GVAII DQWIC AHARC LPTSL SAESG SGGFQ RFFCP) |
| Conjugation | Un-conjugated |
| Alternate Names | FUT13; GDP-fucose protein O-fucosyltransferase 2; C21orf80; Peptide-O-fucosyltransferase 2; O-FucT-2; EC 2.4.1.221 |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purification with Protein A. |
| Buffer | PBS, 0.09% (w/v) Sodium azide and 2% Sucrose. |
| Preservative | 0.09% (w/v) Sodium azide |
| Stabilizer | 2% Sucrose |
| Concentration | Batch dependent: 0.5 - 1 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # Q9Y2G5 Human GDP-fucose protein O-fucosyltransferase 2 |
|---|---|
| Gene Symbol | POFUT2 |
| Gene Full Name | protein O-fucosyltransferase 2 |
| Background | Fucose is typically found as a terminal modification of branched chain glycoconjugates, but it also exists in direct O-linkage to serine or threonine residues within cystine knot motifs in epidermal growth factor (EGF; MIM 131530)-like repeats or thrombospondin (THBS; see MIM 188060) type-1 repeats. POFUT2 is an O-fucosyltransferase that use THBS type-1 repeats as substrates (Luo et al., 2006 [PubMed 16464857]).[supplied by OMIM, Mar 2008] |
| Function | Catalyzes the reaction that attaches fucose through an O-glycosidic linkage to a conserved serine or threonine residue in the consensus sequence C1-X(2,3)-S/T-C2-X(2)-G of thrombospondin type 1 repeats where C1 and C2 are the first and second cysteines, respectively. O-fucosylates members of several protein families including the ADAMTS family, the thrombosporin (TSP) and spondin families. The O-fucosylation of TSRs is also required for restricting epithelial to mesenchymal transition (EMT), maintaining the correct patterning of mesoderm and localization of the definite endoderm (By similarity). Required for the proper secretion of ADAMTS family members such as ADAMSL1 and ADAMST13. [UniProt] |
| Cellular Localization | Endoplasmic reticulum. Golgi apparatus. Note=Mainly located in the endoplasmic reticulum. [UniProt] |
| Calculated MW | 50 kDa |
Images (1) Click the Picture to Zoom In
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ARG42143 anti-POFUT2 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human stomach tissue stained with ARG42143 anti-POFUT2 antibody.
