ARG62617
anti-PODXL antibody [PN-15]
anti-PODXL antibody [PN-15] for Immunohistochemistry,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Rat
Cancer antibody; Cell Biology and Cellular Response antibody; Developmental Biology antibody; Signaling Transduction antibody
Overview
| Product Description | Mouse Monoclonal antibody [PN-15] recognizes PODXL |
|---|---|
| Tested Reactivity | Hu, Rat |
| Tested Application | IHC, IHC-P, WB |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | PN-15 |
| Isotype | IgG1, kappa |
| Target Name | PODXL |
| Antigen Species | Human |
| Immunogen | Microsomal fraction of human renal cortical tissue homogenate. |
| Conjugation | Un-conjugated |
| Alternate Names | Gp200; GCTM-2 antigen; PCLP; Podocalyxin; PC; PCLP-1; Podocalyxin-like protein 1 |
Application Instructions
| Application Note | WB: 1/100 - 1/2000 IHC: 1/10 - 1/500 IHC-P: 1/10 - 1/500 * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
|---|
Properties
| Form | Liquid |
|---|---|
| Purification | Protein G purified |
| Buffer | 10mM PBS (pH 7.4), 0.2% BSA and 0.09% Sodium azide |
| Preservative | 0.09% Sodium azide |
| Stabilizer | 0.2% BSA |
| Concentration | 0.2 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | PODXL |
| Gene Full Name | podocalyxin-like |
| Background | This gene encodes a member of the sialomucin protein family. The encoded protein was originally identified as an important component of glomerular podocytes. Podocytes are highly differentiated epithelial cells with interdigitating foot processes covering the outer aspect of the glomerular basement membrane. Other biological activities of the encoded protein include: binding in a membrane protein complex with Na+/H+ exchanger regulatory factor to intracellular cytoskeletal elements, playing a role in hematopoetic cell differentiation, and being expressed in vascular endothelium cells and binding to L-selectin. [provided by RefSeq, Jul 2008] |
| Function | Involved in the regulation of both adhesion and cell morphology and cancer progression. Function as an anti-adhesive molecule that maintains an open filtration pathway between neighboring foot processes in the podocyte by charge repulsion. Acts as a pro-adhesive molecule, enhancing the adherence of cells to immobilized ligands, increasing the rate of migration and cell-cell contacts in an integrin-dependent manner. Induces the formation of apical actin-dependent microvilli. Involved in the formation of a preapical plasma membrane subdomain to set up inital epithelial polarization and the apical lumen formation during renal tubulogenesis. Plays a role in cancer development and aggressiveness by inducing cell migration and invasion through its interaction with the actin-binding protein EZR. Affects EZR-dependent signaling events, leading to increased activities of the MAPK and PI3K pathways in cancer cells. [UniProt] |
| Research Area | Cancer antibody; Cell Biology and Cellular Response antibody; Developmental Biology antibody; Signaling Transduction antibody |
| Calculated MW | 59 kDa |
| PTM | N- and O-linked glycosylated. Sialoglycoprotein (By similarity). |
Clone References
The utility of PAX-2 in distinguishing metastatic clear cell renal cell carcinoma from its morphologic mimics: an immunohistochemical study with comparison to renal cell carcinoma marker.
IHC / Human
Expression of renal cell carcinoma-associated markers erythropoietin, CD10, and renal cell carcinoma marker in diffuse malignant mesothelioma and metastatic renal cell carcinoma.
IHC / Human
Renal cell carcinoma to the pancreas in surgical pathology material.
IHC / Human
