ARG64235

anti-PODXL antibody

anti-PODXL antibody for Western blot and Human,Mouse,Rat

Cancer antibody; Cell Biology and Cellular Response antibody; Developmental Biology antibody; Signaling Transduction antibody

Overview

Product Description Goat Polyclonal antibody recognizes PODXL
Tested Reactivity Hu, Ms, Rat
Predict Reactivity Dog
Tested Application WB
Specificity This antibody is expected to recognise both reported isoforms (NP_001018121.1 and NP_005388.2).
Host Goat
Clonality Polyclonal
Isotype IgG
Target Name PODXL
Antigen Species Human
Immunogen C-DNLTKDDLDEEEDTH
Conjugation Un-conjugated
Alternate Names Gp200; GCTM-2 antigen; PCLP; Podocalyxin; PC; PCLP-1; Podocalyxin-like protein 1

Application Instructions

Application Suggestion
Tested Application Dilution
WB1 - 3 µg/ml
Application Note WB: Recommend incubate at RT for 1h.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Buffer Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA
Preservative 0.02% Sodium azide
Stabilizer 0.5% BSA
Concentration 0.5 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 192181 Rat PODXL

GeneID: 27205 Mouse PODXL

GeneID: 5420 Human PODXL

Background This gene encodes a member of the sialomucin protein family. The encoded protein was originally identified as an important component of glomerular podocytes. Podocytes are highly differentiated epithelial cells with interdigitating foot processes covering the outer aspect of the glomerular basement membrane. Other biological activities of the encoded protein include: binding in a membrane protein complex with Na+/H+ exchanger regulatory factor to intracellular cytoskeletal elements, playing a role in hematopoetic cell differentiation, and being expressed in vascular endothelium cells and binding to L-selectin. [provided by RefSeq, Jul 2008]
Research Area Cancer antibody; Cell Biology and Cellular Response antibody; Developmental Biology antibody; Signaling Transduction antibody
Calculated MW 59 kDa
PTM N- and O-linked glycosylated. Sialoglycoprotein (By similarity).

Images (1) Click the Picture to Zoom In

  • ARG64235 anti-PODXL antibody WB image

    Western Blot: Human Kisney lysate (35 µg protein in RIPA buffer) stained with ARG64235 anti-PODXL antibody at 1 µg/ml dilution.