ARG55972
anti-PODXL antibody [3D3]
anti-PODXL antibody [3D3] for Flow cytometry,ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Rat
Overview
| Product Description | Mouse Monoclonal antibody [3D3] recognizes PODXL |
|---|---|
| Tested Reactivity | Hu, Rat |
| Tested Application | FACS, ICC/IF, IHC-P, WB |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | 3D3 |
| Isotype | IgG1 |
| Target Name | PODXL |
| Antigen Species | Human |
| Immunogen | Recombinant protein fragment from Human PODXL protein (intracellular, transmembrane, and part of the extracellular domain). |
| Conjugation | Un-conjugated |
| Alternate Names | Gp200; GCTM-2 antigen; PCLP; Podocalyxin; PC; PCLP-1; Podocalyxin-like protein 1 |
Application Instructions
| Application Suggestion |
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| Application Note | IHC-P: Antigen Retrieval: Boil tissue section in 10 mM Citrate buffer (pH 6.0) for 10-20 min, followed by cooling at RT for 20 min. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purification with Protein G. |
| Buffer | PBS (pH 7.4), 0.05% Sodium azide and 0.1 mg/ml BSA |
| Preservative | 0.05% Sodium azide |
| Stabilizer | 0.1 mg/ml BSA |
| Concentration | 0.2 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | PODXL |
| Gene Full Name | podocalyxin-like |
| Background | This gene encodes a member of the sialomucin protein family. The encoded protein was originally identified as an important component of glomerular podocytes. Podocytes are highly differentiated epithelial cells with interdigitating foot processes covering the outer aspect of the glomerular basement membrane. Other biological activities of the encoded protein include: binding in a membrane protein complex with Na+/H+ exchanger regulatory factor to intracellular cytoskeletal elements, playing a role in hematopoetic cell differentiation, and being expressed in vascular endothelium cells and binding to L-selectin. [provided by RefSeq, Jul 2008] |
| Function | Involved in the regulation of both adhesion and cell morphology and cancer progression. Function as an anti-adhesive molecule that maintains an open filtration pathway between neighboring foot processes in the podocyte by charge repulsion. Acts as a pro-adhesive molecule, enhancing the adherence of cells to immobilized ligands, increasing the rate of migration and cell-cell contacts in an integrin-dependent manner. Induces the formation of apical actin-dependent microvilli. Involved in the formation of a preapical plasma membrane subdomain to set up inital epithelial polarization and the apical lumen formation during renal tubulogenesis. Plays a role in cancer development and aggressiveness by inducing cell migration and invasion through its interaction with the actin-binding protein EZR. Affects EZR-dependent signaling events, leading to increased activities of the MAPK and PI3K pathways in cancer cells. [UniProt] |
| Cellular Localization | Cell surface and cytoplasmic |
| Calculated MW | 59 kDa |
| PTM | N- and O-linked glycosylated. Sialoglycoprotein (By similarity). |
Images (4) Click the Picture to Zoom In
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ARG55972 anti-PODXL antibody [3D3] FACS image
Flow Cytometry: NCCIT cells stained with ARG55972 anti-PODXL antibody [3D3] (red) and isotype control antibody (green).
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ARG55972 anti-PODXL antibody [3D3] IHC-P image
Immunohistochemistry: angiosarcoma stained with ARG55972 anti-PODXL antibody [3D3].
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ARG55972 anti-PODXL antibody [3D3] WB image
Western blot: HeLa cell lysate stained with ARG55972 anti-PODXL antibody [3D3].
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ARG55972 anti-PODXL antibody [3D3] IHC-P image
Immunohistochemistry: Formalin-fixed, paraffin-embedded Human placenta stained with ARG55972 anti-PODXL antibody [3D3].
