ARG64482
anti-PNPLA3 / Adiponutrin antibody
anti-PNPLA3 / Adiponutrin antibody for ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse
Cell Biology and Cellular Response antibody; Metabolism antibody; Signaling Transduction antibody
Overview
| Product Description | Goat Polyclonal antibody recognizes PNPLA3 / Adiponutrin |
|---|---|
| Tested Reactivity | Hu, Ms |
| Predict Reactivity | Rat |
| Tested Application | ICC/IF, IHC-P, WB |
| Specificity | This antibody is raised against a peptide derived from the Mouse protein sequence and this peptide differs from Human only in position 2. |
| Host | Goat |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | PNPLA3 / Adiponutrin |
| Antigen Species | Mouse |
| Immunogen | EHDICPKVKSTN |
| Conjugation | Un-conjugated |
| Alternate Names | ADPN; Calcium-independent phospholipase A2-epsilon; iPLA(2)epsilon; Adiponutrin; Patatin-like phospholipase domain-containing protein 3; iPLA2-epsilon; EC 2.3.1.-; C22orf20; EC 3.1.1.3; Acylglycerol O-acyltransferase |
Application Instructions
| Application Suggestion |
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|---|---|---|---|---|---|---|---|---|---|
| Application Note | WB: Recommend incubate at RT for 1h. IHC-P: Antigen Retrieval: Heat mediation was performed in Citrate buffer (pH 6.0). * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Buffer | Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 0.5% BSA |
| Concentration | 0.5 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # Q91WW7 Mouse Patatin-like phospholipase domain-containing protein 3 Swiss-port # Q9NST1 Human Patatin-like phospholipase domain-containing protein 3 |
|---|---|
| Background | The protein encoded by this gene is a triacylglycerol lipase that mediates triacylglycerol hydrolysis in adipocytes. The encoded protein, which appears to be membrane bound, may be involved in the balance of energy usage/storage in adipocytes. [provided by RefSeq, Jul 2008] |
| Research Area | Cell Biology and Cellular Response antibody; Metabolism antibody; Signaling Transduction antibody |
| Calculated MW | 53 kDa |
Images (3) Click the Picture to Zoom In
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ARG64482 anti-PNPLA3 / Adiponutrin antibody ICC/IF image
Immunofluorescence: Paraformaldehyde fixed HepG2 cells permeabilized with 0.15% Triton. Cells were stained with ARG64482 anti-PNPLA3 / Adiponutrin antibody (green) at 10 µg/ml dilution for 1 hour. DAPI (blue) for nuclear staining. Negative control: Unimmunized goat IgG (green) at 10 µg/ml dilution.
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ARG64482 anti-PNPLA3 / Adiponutrin antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human kidney tissue. Antigen Retrieval: Heat mediation was performed in Citrate buffer (pH 6.0). The tissue section was stained with ARG64482 anti-PNPLA3 / Adiponutrin antibody at 8 µg/ml dilution followed by HRP-staining.
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ARG64482 anti-PNPLA3 / Adiponutrin antibody WB image
Western blot: 35 µg of Mouse adrenal gland lysate (in RIPA buffer) stained with ARG64482 anti-PNPLA3 / Adiponutrin antibody at 0.03 µg/ml dilution and incubated at RT for 1 hour.
