ARG64154
anti-PNPLA2 / ATGL antibody
anti-PNPLA2 / ATGL antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human
Cell Biology and Cellular Response antibody; Metabolism antibody; Signaling Transduction antibody
Overview
| Product Description | Goat Polyclonal antibody recognizes PNPLA2 / ATGL |
|---|---|
| Tested Reactivity | Hu |
| Predict Reactivity | Ms, Rat, Cow, Dog, Pig |
| Tested Application | IHC-P, WB |
| Specificity | Pease note this antibody was designed using the Mouse sequence NP_080078.1, which differs by 2 amino acids from the Human sequence NP_065109.1. |
| Host | Goat |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | PNPLA2 / ATGL |
| Antigen Species | Human |
| Immunogen | C-NIIEVSKEARKR |
| Conjugation | Un-conjugated |
| Alternate Names | Transport-secretion protein 2; Pigment epithelium-derived factor; TTS2.2; FP17548; 1110001C14Rik; TTS2; PEDF-R; IPLA2-zeta; ATGL; Calcium-independent phospholipase A2; TTS-2.2; Patatin-like phospholipase domain-containing protein 2; iPLA2zeta; Desnutrin; Adipose triglyceride lipase; EC 3.1.1.3 |
Application Instructions
| Application Suggestion |
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|---|---|---|---|---|---|---|---|
| Application Note | IHC-P: Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). WB: Recommend incubate at RT for 1h. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Buffer | Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 0.5% BSA |
| Concentration | 0.5 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # Q96AD5 Human Patatin-like phospholipase domain-containing protein 2 |
|---|---|
| Background | This gene encodes an enzyme which catalyzes the first step in the hydrolysis of triglycerides in adipose tissue. Mutations in this gene are associated with neutral lipid storage disease with myopathy. [provided by RefSeq, Jul 2010] |
| Research Area | Cell Biology and Cellular Response antibody; Metabolism antibody; Signaling Transduction antibody |
| Calculated MW | 55 kDa |
| PTM | Phosphorylation at Ser-404 by PKA is increased during fasting and moderate intensity exercise, and moderately increases lipolytic activity (By similarity). Phosphorylation at Ser-404 is increased upon beta-adrenergic stimulation. |
Images (2) Click the Picture to Zoom In
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ARG64154 anti-PNPLA2 / ATGL antibody WB image
Western blot: Human Adipose lysate (35 µg protein in RIPA buffer) stained with ARG64154 anti-PNPLA2 / ATGL antibody at 0.5 µg/ml dilution.
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ARG64154 anti-PNPLA2 / ATGL antibody IHC-P image
Immunohistochemistry: Paraffin embedded Human Heart. (Steamed antigen retrieval with citrate buffer pH 6) stained with ARG64154 anti-PNPLA2 / ATGL antibody at 2.5 µg/ml dilution followed by AP-staining.
