ARG63156

anti-PLRG1 antibody

anti-PLRG1 antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse

Gene Regulation antibody

Overview

Product Description Goat Polyclonal antibody recognizes PLRG1
Tested Reactivity Hu, Ms
Predict Reactivity Cow, Rat, Dog, Pig
Tested Application IHC-P, WB
Specificity This antibody is expected to recognise isoform 1 (NP_002660.1) and isoform 2 (NP_001188493.1).
Host Goat
Clonality Polyclonal
Isotype IgG
Target Name PLRG1
Antigen Species Human
Immunogen C-PVSWKPEIIKRKRF
Conjugation Un-conjugated
Alternate Names PRL1; Pleiotropic regulator 1; PRP46; TANGO4; PRPF46; Cwc1

Application Instructions

Application Suggestion
Tested Application Dilution
IHC-P5 µg/ml
WB0.1 - 0.3 µg/ml
Application Note WB: Recommend incubate at RT for 1h.
IHC-P: Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0).
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Buffer Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA
Preservative 0.02% Sodium azide
Stabilizer 0.5% BSA
Concentration 0.5 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 53317 Mouse PLRG1

GeneID: 5356 Human PLRG1

Swiss-port # O43660 Human Pleiotropic regulator 1

Swiss-port # Q922V4 Mouse Pleiotropic regulator 1

Background This gene encodes a core component of the cell division cycle 5-like (CDC5L) complex. The CDC5L complex is part of the spliceosome and is required for pre-mRNA splicing. The encoded protein plays a critical role in alternative splice site selection. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Jan 2011]
Research Area Gene Regulation antibody
Calculated MW 57 kDa

Images (2) Click the Picture to Zoom In

  • ARG63156 anti-PLRG1 antibody IHC-P image

    Immunohistochemistry: Paraffin-embedded Human prostate tissue. Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). The tissue section was stained with ARG63156 anti-PLRG1 antibody at 5 µg/ml dilution followed by AP-staining.

  • ARG63156 anti-PLRG1 antibody WB image

    Western blot: 35 µg of HEK293 (A) and NIH/3T3 (B) nuclear lysates (in RIPA buffer) stained with ARG63156 anti-PLRG1 antibody at 0.1 µg/ml dilution and incubated at RT for 1 hour.