ARG52397
anti-PLK1 phospho (Ser482 / Ser486 / Ser490) antibody
anti-PLK1 phospho (Ser482 / Ser486 / Ser490) antibody for Western blot and Xenopus laevis
Cancer antibody; Cell Biology and Cellular Response antibody; Gene Regulation antibody
Overview
| Product Description | Rabbit Polyclonal antibody recognizes PLK1 phospho (Ser482 / Ser486 / Ser490) |
|---|---|
| Tested Reactivity | Xenopus laevis |
| Predict Reactivity | Hu, Ms, Rat, Zfsh |
| Tested Application | WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | PLK1 |
| Antigen Species | Xenopus laevis |
| Immunogen | Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser482/486/490 conjugated to KLH |
| Conjugation | Un-conjugated |
| Alternate Names | STPK13; Serine/threonine-protein kinase PLK1; EC 2.7.11.21; Serine/threonine-protein kinase 13; Polo-like kinase 1; PLK-1; PLK |
Application Instructions
| Application Suggestion |
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| Application Note | Specific for the ~120k PLKK protein phosphorylated at Ser482,486,490. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity Purified |
| Buffer | 10 mM HEPES (pH 7.5), 150 mM NaCl, 0.1 mg/ml BSA and 50% Glycerol |
| Stabilizer | 0.1 mg/ml BSA, 50% Glycerol |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
GeneID: 380481 Xenopus laevis PLK1 Swiss-port # P70032 Xenopus laevis Serine/threonine-protein kinase PLK1 |
|---|---|
| Gene Symbol | PLK1 |
| Gene Full Name | polo-like kinase 1 |
| Background | Considerable evidence indicates that a polo-like kinase (PLK) plays an important role in cell cycle regulation. PLK is also required for bipolar spindle formation, activation of the anaphase-promoting complex/cyclosome, and cytokinesis. Recent work led to the identification of a PLKK that is thought to be responsible for activation of PLK. Recent work (Erikson, et al., 2004) has shown that PLKK is in turn activated by phosphorylation at three sites (Ser482, Ser486 and Ser490). Thus activation of PLK is thought to involve a kinase cascade involving the phosphorylation of Ser482,486,490 in PLKK. |
| Research Area | Cancer antibody; Cell Biology and Cellular Response antibody; Gene Regulation antibody |
| Calculated MW | 68 kDa |
| PTM | Catalytic activity is enhanced by phosphorylation of Thr-210. Phosphorylation at Thr-210 is first detected on centrosomes in the G2 phase of the cell cycle, peaks in prometaphase and gradually disappears from centrosomes during anaphase. Dephosphorylation at Thr-210 at centrosomes is probably mediated by protein phosphatase 1C (PP1C), via interaction with PPP1R12A/MYPT1. Autophosphorylation and phosphorylation of Ser-137 may not be significant for the activation of PLK1 during mitosis, but may enhance catalytic activity during recovery after DNA damage checkpoint. Phosphorylated in vitro by STK10. Ubiquitinated by the anaphase promoting complex/cyclosome (APC/C) in anaphase and following DNA damage, leading to its degradation by the proteasome. Ubiquitination is mediated via its interaction with FZR1/CDH1. Ubiquitination and subsequent degradation prevents entry into mitosis and is essential to maintain an efficient G2 DNA damage checkpoint. Monoubiquitination at Lys-492 by the BCR(KLHL22) ubiquitin ligase complex does not lead to degradation: it promotes PLK1 dissociation from phosphoreceptor proteins and subsequent removal from kinetochores, allowing silencing of the spindle assembly checkpoint (SAC) and chromosome segregation. |
